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Chromatographia

, Volume 72, Issue 7–8, pp 627–633 | Cite as

Simultaneous Determination of Levodopa, Benserazide and 3-O-Methyldopa in Human Serum by LC–MS–MS

Original

Abstract

For the first time a sensitive, specific and rapid LC–MS–MS assay is presented for the simultaneous determination of levodopa (L-DP), 3-O-methyldopa (3-OMD) and benserazide (BSZ) in human serum. The three compounds were extracted from human serum by protein precipitation followed by dilution of the supernatant with aqueous formic acid. In serum, linearity was observed between 50 and 1,000 ng mL−1 of L-DP, 3-OMD and BSZ, respectively. Intra-day and inter-day RSD values were below 10.56 and 6.22% at concentrations of 120, 360 and 720 ng mL−1. The presented method showed excellent specificity and sensitivity compared with other methods reported. It was applied to a pharmacokinetic study and demonstrated its applicability to pre-clinical and clinical pharmacological research.

Keywords

Column liquid chromatography-tandem mass spectrometry Levodopa Benserazide 3-O-methyldopa 

References

  1. 1.
    Muzzi C, Bertocci E, Terzuoli L, Porcelli B, Ciari I, Pagani R, Guerranti R (2008) Biomed Pharmacother 62:253–258CrossRefGoogle Scholar
  2. 2.
    Marques KL, Santos JL, Lopes JA, Lima JL (2008) Anal Sci 24:985–991CrossRefGoogle Scholar
  3. 3.
    Nunes T, Machado R, Rocha JF, Fernandes-Lopes C, Costa R, Torrão L, Loureiro AI, Falcão A, Vaz-da-Silva M, Wright L, Almeida L, Soares-da-Silva P (2009) Clin Ther 31:2258–2271CrossRefGoogle Scholar
  4. 4.
    Blandini F, Martignoni E, Pacchetti C, Desideri S, Rivellini D, Nappi G (1997) J Chromatogr B Anal Technol Biomed Life Sci 700:278–282CrossRefGoogle Scholar
  5. 5.
    Michotte Y, Moors M, Deleu D, Herregodts P, Ebinger G (1987) J Pharm Biomed Anal 5:659–664CrossRefGoogle Scholar
  6. 6.
    Igarashi K, Hotta K, Kasuya F, Abe K, Sakoda S (2003) J Chromatogr B Anal Technol Biomed Life Sci 792:55–61CrossRefGoogle Scholar
  7. 7.
    Davidson AG (1985) J Pharm Biomed Anal 3:235–240CrossRefGoogle Scholar
  8. 8.
    Hassib ST (1990) Anal Lett 23:2195–2214Google Scholar
  9. 9.
    Uslua B, Özkana SA (2002) Anal Lett 35:303–314CrossRefGoogle Scholar
  10. 10.
    Karpińska J, Smyk J, Wołyniec E (2005) Spectrochim Acta A 62:213–220CrossRefGoogle Scholar
  11. 11.
    Pistonesi M, Centurión ME, Fernández Band BS, Damiani PC, Olivieri AC (2004) J Pharm Biomed Anal 36:541–547CrossRefGoogle Scholar
  12. 12.
    Coello J, Maspoch S, Villegas N (2000) Talanta 53:627–637CrossRefGoogle Scholar
  13. 13.
    Wu G, Furlanut M (1997) Pharmacol Res 35:553–556CrossRefGoogle Scholar
  14. 14.
    Andreoli R, Manini P, Bergamaschi E, Mutti A, Franchini I, Niessen WM (1999) J Chromatogr A 847:9–17CrossRefGoogle Scholar
  15. 15.
    Lee MS, Kerns EH (1999) Mass Spectrom Rev 18:187–279CrossRefGoogle Scholar
  16. 16.
    Crevoisier C, Monrealc A, Metzgerc NilsenT (2003) Eur Neurol 49:39–44CrossRefGoogle Scholar
  17. 17.
    Zhang D, Wang Y, Han J, Yu W, Deng L, Fawcett JP, Liu Z, Gu J (2009) J Chromatogr B Anal Technol Biomed Life Sci 877:581–585CrossRefGoogle Scholar
  18. 18.
    Juan-García A, Font G, Picó Y (2007) Electrophoresis 28:4180–4191CrossRefGoogle Scholar
  19. 19.
    Yüzüak N, Özden T, Erenl S, Toptan S (2007) Chromatographia 66:115–118CrossRefGoogle Scholar
  20. 20.
    Damen CW, Rosing H, Tibben MM, van Maanen MJ, Lagas JS, Schinkel AH, Schellens JH, Beijnen JH (2008) J Chromatogr B Anal Technol Biomed Life Sci 868:102–109CrossRefGoogle Scholar
  21. 21.
    Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030CrossRefGoogle Scholar

Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  1. 1.School of PharmacyFudan UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Pharmacy, Huashan HospitalFudan UniversityShanghaiPeople’s Republic of China

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