, Volume 72, Issue 5–6, pp 431–440 | Cite as

Global Chemome Study by LC Coupled with DAD and ESI–Q–TOF MS of a Composite Traditional Chinese Medicine Qishenyiqi Dropping Pills

  • Jing Wang
  • Na Wei
  • Hongzhi Zhao
  • Hao Huang
  • Min Jiang
  • Gang Bai
  • Guoan Luo


High-performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole-time of flight mass spectrometry (ESI–Q–TOF MS) has been used for global chemome analysis of Qishenyiqi dropping pills. Under gradient conditions, two sub-chemomes extracted from Qishenyiqi dropping pills were analyzed on the same C18 reversed-phase column by use of different binary mobile phases. In the chromatogram obtained from sub-chemome I, 19 compounds with strong ultraviolet absorption at 260 nm were detected; 27 compounds with weak ultraviolet absorption at 203 nm were detected in the chromatogram obtained from sub-chemome II. All the compounds were identified by ESI–Q–TOF MS. This study furnished much more important information about the pharmacological activity of Qishenyiqi dropping pills than previous studies and laid a good foundation for further exploration of chemomics.


Column liquid chromatography DAD–Q–TOF mass spectrometry Chemomics Global chemome Sub-chemome Traditional Chinese medicine prescription 



This study was supported financially by the National Key Special Project of Science and Technology for Innovation Drugs of China (grant no. 2008ZX09401-006). The authors are grateful for this financial support. The authors also acknowledge Tianjin Tasly Pharmaceutical Co. Ltd. for providing the Qishenyiqi dropping pills.


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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  1. 1.College of PharmacyNankai UniversityTianjinPeople’s Republic of China
  2. 2.Department of ChemistryTsinghua UniversityBeijingPeople’s Republic of China

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