Global Chemome Study by LC Coupled with DAD and ESI–Q–TOF MS of a Composite Traditional Chinese Medicine Qishenyiqi Dropping Pills
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High-performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole-time of flight mass spectrometry (ESI–Q–TOF MS) has been used for global chemome analysis of Qishenyiqi dropping pills. Under gradient conditions, two sub-chemomes extracted from Qishenyiqi dropping pills were analyzed on the same C18 reversed-phase column by use of different binary mobile phases. In the chromatogram obtained from sub-chemome I, 19 compounds with strong ultraviolet absorption at 260 nm were detected; 27 compounds with weak ultraviolet absorption at 203 nm were detected in the chromatogram obtained from sub-chemome II. All the compounds were identified by ESI–Q–TOF MS. This study furnished much more important information about the pharmacological activity of Qishenyiqi dropping pills than previous studies and laid a good foundation for further exploration of chemomics.
KeywordsColumn liquid chromatography DAD–Q–TOF mass spectrometry Chemomics Global chemome Sub-chemome Traditional Chinese medicine prescription
This study was supported financially by the National Key Special Project of Science and Technology for Innovation Drugs of China (grant no. 2008ZX09401-006). The authors are grateful for this financial support. The authors also acknowledge Tianjin Tasly Pharmaceutical Co. Ltd. for providing the Qishenyiqi dropping pills.
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