Skip to main content
SpringerLink
Log in

LC–MS–MS Simultaneous Determination of l-Dopa and Its Prodrug l-Dopa n-Pentyl Ester Hydrochloride in Rat Plasma

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A sensitive, simple and rapid LC–MS–MS method has been developed and validated for the simultaneous determination of l-dopa and l-dopa n-pentyl ester hydrochloride in rat plasma in the present study. The analytes were separated on a C18 column (5 μm, 2.1 × 150 mm) with a security guard C18 column (5 μm, 4 × 20 mm) and a triple-quadrupole mass spectrometer was applied for detection. The method was linear over the concentration ranges of 25–5,000 ng mL−1 for l-dopa and 12.5–2,500 ng mL−1 for l-dopa n-pentyl ester hydrochloride. Finally, the method was successfully applied to support the pharmacokinetic study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Serra PA, Esposito G, Enrico P, Mura MA, Migheli R, Delogu MR, Miele M, Desole MS, Grella G, Miele E (2000) Br J Pharmacol 130:937–945

    Article  CAS  Google Scholar 

  2. Tofaris GK, Spillantini MG (2005) Mov Disord 20:S37–S44

    Article  Google Scholar 

  3. Cingolani GM, Stefano AD, Mosciatti B, Napolitani F, Giorgionic G, Ricciutelli M, Claudi F (2000) Bioorg Med Chem Lett 10:1385–1388

    Article  CAS  Google Scholar 

  4. Dethy S, Laute MA, Van Blercom NV, Damhaut PD, Goldman S, Hildebrand J (1997) Clin Chem 43:740–744

    CAS  Google Scholar 

  5. Blandini F, Martignoni E, Pacchetti C, Desideri S, Rivellini D, Nappi G (1997) J Chromatogr B700:278–282

    Google Scholar 

  6. Rondelli I, Acerbi D, Mariotti F, Ventura P (1994) J Chromatogr B 653:17–23

    Article  CAS  Google Scholar 

  7. Wikberg T (1991) J Pharm Biomed Anal 9:167–176

    Article  CAS  Google Scholar 

  8. Igarashi K, Hotta K, Kasuya F, Abe K, Sakoda S (2003) J Chromatogr B 792:55–61

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was partly supported by the National Natural Science Foundation of China (30760309), Guangxi Natural Science Foundation (0832014Z) and Innovation Project of Guangxi Graduate Education (2008105981007M195).

Author information

Authors and Affiliations

  1. School of Pharmaceutical, Guangxi Medical University, 530021, Nanning, People’s Republic of China

    Li Lv, Weizhe Jiang, Songyu Zhou, Xingzhen Huang, Xiaoxia Shi, Cong Lv, Lingling Wu & Chongyao Xu

Authors
  1. Li Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weizhe Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Songyu Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xingzhen Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaoxia Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cong Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lingling Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chongyao Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Weizhe Jiang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lv, L., Jiang, W., Zhou, S. et al. LC–MS–MS Simultaneous Determination of l-Dopa and Its Prodrug l-Dopa n-Pentyl Ester Hydrochloride in Rat Plasma. Chroma 72, 239–243 (2010). https://doi.org/10.1365/s10337-010-1668-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-010-1668-9

Keywords

Search

Navigation