Chromatographia

, Volume 72, Issue 3–4, pp 357–359 | Cite as

Ion-pair LC–UV Method for the Determination of Boanmycin in Mouse Plasma and Its Application to a Pharmacokinetic Study

  • Yu Liu
  • Sun Dong Yoo
  • Liang Fang
  • Xin Jin
  • Li Li
  • Tiefu Li
Limited Short Communication

Abstract

An ion-pair LC–UV detection assay was developed for the determination of boanmycin (BAM) in mouse plasma. The mobile phase contained methanol, acetonitrile, 0.055 M sodium hexanesulfonate and 0.08 M acetic acid in water, with a flow rate of 0.8 mL min−1. This assay involved a rapid and simple extraction process and subsequent detection at 291 nm. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 and 0.09 μg mL−1, respectively. Using bleomycin (BLM A5) as the internal standard, the assay was linear over the concentration range from 0.1 to 30 μg mL−1 (r 2 > 0.99). The extraction recovery of BAM in mouse plasma was >66.5%, and the intra- and inter-day accuracy and precision were ≤7.5%. The assay was successfully applied to a pharmacokinetic study after intravenous injection of BAM (40 mg kg−1) to mice.

Keywords

Column liquid chromatography Ion-pair chromatography Pharmacokinetics study Sodium hexanesulfonate Boanmycin in mouse plasma 

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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Yu Liu
    • 1
  • Sun Dong Yoo
    • 2
  • Liang Fang
    • 1
  • Xin Jin
    • 1
  • Li Li
    • 1
  • Tiefu Li
    • 3
  1. 1.School of PharmacyShenyang Pharmaceutical UniversityShenyangChina
  2. 2.School of PharmacySungkyunkwan UniversitySuwonKorea
  3. 3.School of Pharmaceutical EngineeringShenyang Pharmaceutical UniversityShenyangChina

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