Skip to main content
SpringerLink
Log in

LC Simultaneous Determination of Thioctic Acid, Benfotiamine and Cyanocobalamin in Thiotacid Compound Capsules

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A simple, selective, sensitive, precise, simultaneous liquid chromatographic analysis of capsules containing thioctic acid, benfotiamine and cyanocobalamin was described. Good chromatographic separation was achieved using a Zorbax C18 (4.6 cm × 250 mm, 5 μm) and a mobile phase consisting of acetonitrile–phosphate buffer pH 3.5 (15:85, v/v) at a flow rate of 0.9 mL min−1. The ultraviolet detector was set a wavelength of 280 nm. Thioctic acid, benfotiamine and cyanocobalamin were eluted at 2.869, 3.752 and 13.689 min, respectively. The linear ranges for thioctic acid, benfotiamine and cyanocobalamin were 30–180, 4–24 and 0.025–0.150 μg mL−1, respectively. The recoveries of thioctic acid, benfotiamine and cyanocobalamine in pharmaceutical preparation were all greater than 98% and their relative standard deviations were less than 2.0%. The limits of detection were 2.57, 0.19 and 0.003 μg mL−1 for thioctic acid, benfotiamine and cyanocobalamin, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Reynolds JEF (ed) (1982) Martindale: the extra Pharmacopoeia, 28th edn. The Pharmaceutical Press, London

  2. Merck Index, 12th edn (1995) Merck & Co. Inc., White House Station

  3. Koricanac Z, Cakar M, Tanaskovic S, Jovanovic T (2007) J Serb Chem Soc 72:29–35

    Article  CAS  Google Scholar 

  4. Ibrahim F, Ali F, Ahmed S, Tolba M (2007) J Chin Chem Soc 54:365–374

    CAS  Google Scholar 

  5. El-Enany N, Belal F, Rizk M (2007) J Chin Chem Soc 54:941–948

    CAS  Google Scholar 

  6. Ibrahim F, Ali F, Ahmed S, Tolba M (2007) J Chin Chem Soc 54:925–932

    CAS  Google Scholar 

  7. Jackman S, Hough D, Danson M, Stevenson K, Opperdoes F (1990) J Biochem 193:91

    CAS  Google Scholar 

  8. Chen J, Jiang W, Cai J, Tao W, Gao X, Jiang X (2005) J Chromatogr B 824:249–250

    Article  CAS  Google Scholar 

  9. Teichert J, Preiss R (2002) J Chromatogr B 769:269

    Article  CAS  Google Scholar 

  10. Haj-yehia A, Assaf P, Nassar T, Katzhendler J (2000) J Chromatogr A 870:381–383

    Article  CAS  Google Scholar 

  11. The British Pharmacopoeia (2007) Her Majesty’s Stationary Office, London, 456, 1649

  12. Hoogvliet J, Dijksma M, Kamp B, Van-Bennekom W (2000) Anal Chem 72:2016–2017

    Article  CAS  Google Scholar 

  13. Gadzekpo V, Xiao K, Aoki H, Buhlmann P, Umezawa Y (1999) Anal Chem 71:5109–5200

    Article  CAS  Google Scholar 

  14. Sitton A, Schmid M, Gubit Z, Aboul-Enein H (2004) J Biochem Biophys Methods 61:119

    Article  CAS  Google Scholar 

  15. Lin J, Alt A, Liersch J, Bretzel R, Brownlee M (2000) Diabetes 49:A143

    Article  Google Scholar 

  16. Balakumar P, Sharma R, Singh M (2008) Pharm Res 58:356–363

    Article  CAS  Google Scholar 

  17. Sanchez-Ramirez G, Caram-Salas N, Rocha-Gonzalez H, Vidal-Cantu G, Medina-Santillan R, Reyes-Garcia G, Granados-Soto V (2006) Eur J Pharm 530:48–53

    Article  CAS  Google Scholar 

  18. Maybodi A, Hassani S (2008) Spectro Acta A 70:1167–1172

    Article  Google Scholar 

  19. Sena M, Chaudhry Z, Collins C, Poppi R (2004) J Pharm Biomed Anal 36(4):743–749

    Article  CAS  Google Scholar 

  20. Nepote A, Damiani P, Olivieri A (2003) J Pharm Biomed Anal 3:621–627

    Article  Google Scholar 

  21. Hou H, Qi Z, Yang Y, Liao F, Zhang Y, Liu Y (2008) J Pharm Biomed Anal 47:134–139

    Article  CAS  Google Scholar 

  22. Xu H, Li Y, Liu C, Wu Q, Zhao Y, Lu L, Tang H (2008) Talanta 77:176–181

    Article  CAS  Google Scholar 

  23. Hernández S, Ribero G, Goicoechea H (2003) Talanta 61(5):743–753

    Article  Google Scholar 

  24. Riccio F, Mennella C, Fogliano V (2006) J Pharm Biomed Anal 41:1592–1595

    Article  CAS  Google Scholar 

  25. Heudi O, Kilinç T, Fontannaz P (2005) J Chromatogr A 1070:49–56

    Article  CAS  Google Scholar 

  26. Li L, Da S, Feng Y, Liu M (2004) Talanta 64:373–379

    Article  CAS  Google Scholar 

  27. Viñas P, López-Erroz C, Balsalobre N, Hernández-Córdoba M (2003) J Chromatogr A 1007:77–84

    Article  Google Scholar 

  28. Markopoulou C, Kagkadis K, Koundourellis J (2002) J Pharm Biomed Anal 30:1403–1410

    Article  CAS  Google Scholar 

  29. Moreno P, Salvadó V (2000) J Chromatogr A 870:207–215

    Article  CAS  Google Scholar 

  30. Wongyai S (2000) J Chromatogr A 870:217–220

    Article  CAS  Google Scholar 

  31. Lebiedzińska A, Marszałł M, Kuta J, Szefer P (2007) J Chromatogr A 1173:71–80

    Article  Google Scholar 

  32. Luo X, Chen B, Ding L, Tang F, Yao S (2006) Anal Chim Acta 562:185–189

    Article  CAS  Google Scholar 

  33. Guidance for Industry; Q2B of analytical procedures: methodology; International Conference on Harmonization (ICH), November 1996. http:/www.fda.gov/eder/guidance/1320fnl.pdf. Accessed 1 Sept 2004

Download references

Author information

Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Science and Arts (MSA University), October 6, Egypt

    Hesham Salem

Authors
  1. Hesham Salem
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hesham Salem.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Salem, H. LC Simultaneous Determination of Thioctic Acid, Benfotiamine and Cyanocobalamin in Thiotacid Compound Capsules. Chroma 72, 327–330 (2010). https://doi.org/10.1365/s10337-010-1635-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-010-1635-5

Keywords

Search

Navigation