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Determination of Propylenethiourea, the Main Metabolite of Propineb, in Tomato by HILIC-MS

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Abstract

A quantitative acetonitrile extraction with dispersive solid-phase clean-up and subsequent high performance hydrophilic interaction liquid chromatography-atmospheric pressure electrospray ionization mass spectrometry (HILIC-ESI-MS-MS) analysis is outlined for the determination of propylenethiourea (PTU, 4-methylimidazolidine-2-thione), the main degradation product of propylenebisdithiocarbamate (PBDC). In the present study, the quick, easy, cheap, effective, rugged and safe method (QuEChERS) methodology was adopted for the extraction of PTU from tomato matrix. The synthesis and impurity profiling of a new internal standard compound (4,5-dimethylimidazolidine-2-thione) is also reported, which was used instead of tris(1,3-dichlorisopropyl)phosphate due to poor ionization at elevated acetonitrile levels. The effect of chromatographic parameters on the HILIC separation and MS detection was studied. Coupling of hydrophilic interaction liquid chromatography with mass spectrometric detection was advantageous not only in view of separation and ionization efficiency but also in direct injection of the QuEChERS extract without the need for solvent exchange.

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Acknowledgments

We gratefully acknowledge Kromat Ltd. for providing the LC–MS-MS system and Wessling Hungary Ltd. for their excellent cooperation. Thanks are also due to Mr. S. Bilsborough for the useful comments on the manuscript.

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Authors and Affiliations

  1. Joint Research and Training Laboratory on Separation Techniques, Eötvös Loránd University, Pázmány P. s. 1/a, Budapest, 1117, Hungary

    László Tölgyesi & Kornél Torkos

  2. Kromat Ltd. Co., Péterhegyi u. 98, Budapest, 1112, Hungary

    László Tölgyesi

  3. Institute of Chemistry, Eötvös Loránd University, Pázmány P. s. 1/a, Budapest, 1117, Hungary

    Péter Kele

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  1. László Tölgyesi
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Correspondence to László Tölgyesi.

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Tölgyesi, L., Kele, P. & Torkos, K. Determination of Propylenethiourea, the Main Metabolite of Propineb, in Tomato by HILIC-MS. Chroma 71 (Suppl 1), 75–80 (2010). https://doi.org/10.1365/s10337-010-1617-7

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