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LC Determination of the Intestinal Absorption of Etoposide in Vitro and in Rat Plasma

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Abstract

Etoposide (VP16), an anti-neoplastic agent, exhibits low and variable bioavailability. We have developed and validated a rapid, sensitive, and accurate LC–UV method for the determination of VP16 in vitro and in rat plasma after oral administration. The method was then used to investigate the absorption characteristics of VP16 with an in vitro diffusion chamber system across isolated rat intestinal membranes as a model. Borneol and eugenol were used to enhance the intestinal absorption of VP16. We also examined the effect of borneol and eugenol on the intestinal absorption of VP16 in vivo. The results showed that the plasma concentration of VP16 was significantly increased; relative bioavailability was 2.2-fold and 2.9-fold enhancement in the presence of borneol and eugenol, respectively, compared to VP16 administered alone.

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Acknowledgments

The work was supported by the Shanghai Municiple Committee of Science and Technology (Grant No. 08DZ1971304), the National Basic Research Program of China (973 Program), No.2007CB936004, and the Key Projects of National New Drug 2009ZX09502-009.

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China

    Tiecheng Zhang & Weisan Pan

  2. School of Pharmacy, Shanghai JiaoTong University, Dongchuan Road 800, Shanghai, 200240, China

    Tiecheng Zhang & Qi Shen

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  1. Tiecheng Zhang
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  2. Qi Shen
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  3. Weisan Pan
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Correspondence to Qi Shen.

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Zhang, T., Shen, Q. & Pan, W. LC Determination of the Intestinal Absorption of Etoposide in Vitro and in Rat Plasma. Chroma 71, 993–998 (2010). https://doi.org/10.1365/s10337-010-1593-y

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  • DOI: https://doi.org/10.1365/s10337-010-1593-y

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