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Rapid RP-LC Method with Fluorescence Detection for Analysis of Fexofenadine in Human Plasma

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Abstract

A rapid and sensitive reversed-phase high-performance liquid chromatographic method for analysis of fexofenadine in human plasma has been developed and optimized. The analytes were extracted from biological samples by solid-phase extraction on hydrophilic–lipophilic balance cartridges. LC separation was performed on a C18 analytical column (125 mm × 4 mm i.d., 5-μm particles) with 42:58 (v/v) acetonitrile–water adjusted to pH 2.7 with 85% orthophosphoric acid as mobile phase. Fluorescence detection was performed with excitation at 230 nm and emission at 290 nm. The total time for chromatographic separation was 7 min. The method was validated in accordance with EU guidelines by analysis of plasma samples fortified with fexofenadine at concentrations between 0.05 and 800 ng mL−1. Calibration plots were linear in this range. Mean recovery was typically 94.03% and the detection limit was 0.05 ng mL−1. The time required for quantitative analysis is shorter than that required by other methods.

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References

  1. Fromm MF (2000) Int J Clin Pharmacol Ther 38:69–74. PMID 10706193

    Google Scholar 

  2. Kim RB, Leake BF, Choo EF, Dresser GK, Kubba SV, Schwarz UI, Taylor A, Xie HG, McKinsey J, Zhou S, Lan 3. LB, Schultz JD, Schultz EG (2001) Clin Pharmacol Ther 70:189–199. PMID 11503014

  3. Orzechowski RF, Currie DS, Valancius CA (2005) Eur J Pharmacol 506:257–264. doi:10.1016/j.ejphar.2004.11.006

    Article  CAS  Google Scholar 

  4. Liu H, Farley JM (2005) BMC Pharmacol 5:2–10. doi:10.1186/1471-2210-5-8

  5. Borade PS, Ballary CC, Currie GP, Lee DKC (2006) Ther Strateg 3:253–259. doi:10.1016/j.ddstr.2006.08.005

    Article  Google Scholar 

  6. Kawashima M, Harada S (2007) J Dermatol 34:9–16. doi:10.1111/j.1346-8138.2007.00209.x

    Google Scholar 

  7. Howarth PH (2002) Clin Exp Allergy Rev 2:18–25. doi:10.1046/j.1472-9725.2002.00030.x

  8. Bachert C (2009) Clin Ther 31:921–944. doi:10.1016/j.clinthera.2009.05.017

    Article  CAS  Google Scholar 

  9. Pechadre JC, Beudin P, Eschalier A, Trolese JF, Rihoux JP (1991) J Int Med Res 19:289–295. doi:10.1002/hup.538. http://www.ncbi.nlm.nih.gov/pubmed/1680760

    Google Scholar 

  10. Mason J, Reynolds R, Rao N (1999) Clin Exp Allergy 29:163–170. doi:10.1046/j.1365-2222.1999.0290s3163.x

    Google Scholar 

  11. Breier AR, Steppe M, Schapoval EES (2006) Chromatographia 64:725–729. doi:10.1365/s10337-006-0096-3

    Google Scholar 

  12. Karakus S, Kucukguzel I, Kucukguzel SG (2008) J Pharm Biomed Anal 46:295–302. doi:10.1016/j.jpba.2007.10.018

    Article  CAS  Google Scholar 

  13. Miura M, Uno T, Tateishi T, Suzuki T (2007) J Pharm Biomed Anal 43:741–745. doi:10.1016/j.jpba.2006.07.033

    Article  CAS  Google Scholar 

  14. Coutant JE, Westmark PA, Nardella PA, Walter SM, Okerholm RA (1991) J Chromatogr 570:139–148

    Google Scholar 

  15. Surapaneni S, Khalil SKW (1996) J Liq Chromatogr Biomed Anal 17:2419–2428. doi:10.1016/0731-7085(95)01713-5

    Google Scholar 

  16. Hofmann U, Seiler M, Drescher S, Fromm MF (2002) J Chromatogr B 766:227–233. doi:10.1016/S0378-4347(01)00468-6

    Article  CAS  Google Scholar 

  17. Yamane N, Tozuka Z, Sugiyama Y, Tanimoto T, Yamazaki A, Kumagai Y (2007) J Chromatogr B 858:118–128. doi:10.1016/j.jchromb.2007.08.011

    Article  CAS  Google Scholar 

  18. Uno T, Y-Furukori N, Takahata T, Sugawara K, Tateishi T (2004) J Pharm Biomed Anal 35:937–942. doi:10.1016/j.jpba.2004.02.036

    Article  CAS  Google Scholar 

  19. Breier AR, Garcia SS, Jabłoński A, Steppie M, Schapoval ES (2005) J AOAC Int 88:1059–1063

    Google Scholar 

  20. Mikus P, Vlaskova I, Hawranek E (2005) Drug Dev Ind Pharm 31:795–801. doi:10.1080/03639040500217368

    Google Scholar 

  21. Isleyen E, Ozden T, Ozilhan S, Tophan S (2007) Chromatographia 66:s109–s113. doi:10.1365/s10337-007-0267-x

  22. Ramenskaya G, Skuridina E, Krasnykh M (2006) Pharm Chem J 40:686–689. doi:10.1007/s11094-006-0220-6

    Google Scholar 

  23. Nirogi RVS, Kandikere VN, Shukla M, Mudigonda K, Maurya S, Komarneni P (2007) Biomed Chromatogr 21:209–216. doi:10.1002/bmc.740

    Google Scholar 

  24. Fu I, Woolf E, Matuszewski B (2004) J Pharm Biomed Anal 35:837–846. doi:10.1016/j.jpba.2004.02.016

    Article  CAS  Google Scholar 

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Correspondence to Lucyna Konieczna.

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Konieczna, L., Plenis, A., Olędzka, I. et al. Rapid RP-LC Method with Fluorescence Detection for Analysis of Fexofenadine in Human Plasma. Chroma 71, 1081–1086 (2010). https://doi.org/10.1365/s10337-010-1592-z

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  • DOI: https://doi.org/10.1365/s10337-010-1592-z

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