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Extraction and Determination of β-Sitosterol from Salicornia herbacea L. Using Monolithic Cartridge

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Abstract

A short ionic liquids-based monolithic cartridge was prepared and used as the selective extraction sorbent. Characteristic and evaluation are investigated by field emission scanning electron microscopy (FE-SEM), and a new approach was developed for the extraction and determination of β-sitosterol from Salicornia herbacea L. using the ionic liquids-based monolithic cartridge. Chromatographic analysis was conducted on a C18 column with UV detection at 210 nm, and an eluting solution consisting of acetonitrile–water (60/40, v/v) was used as the mobile phase at a flow rate of 0.8 mL min−1. The linearity was confirmed in the concentration range of 0.50–100.00 μg mL−1, with RSDs within 4.20%, and a recovery of β-sitosterol ranging from 97.20 to 102.93%. This method effectively removed the impurities without any tedious pretreatment, and it provided a fast, economic and effective way to assay trace drugs from natural plants.

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Acknowledgment

This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2009-0072787).

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Authors and Affiliations

  1. Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon, 402-751, Korea

    Tao Zhu & Kyung Ho Row

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  1. Tao Zhu
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  2. Kyung Ho Row
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Correspondence to Kyung Ho Row.

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Zhu, T., Row, K.H. Extraction and Determination of β-Sitosterol from Salicornia herbacea L. Using Monolithic Cartridge. Chroma 71, 981–985 (2010). https://doi.org/10.1365/s10337-010-1574-1

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  • DOI: https://doi.org/10.1365/s10337-010-1574-1

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