, Volume 71, Issue 9–10, pp 907–911 | Cite as

Four-Dimensional TLC on Plates with Open and Closed Adsorbent Layers

  • Victor Berezkin
  • Svetlana Khrebtova
  • Natalia Kulakova


Four-stage TLC, a new version of multidimensional (multi-stage) planar chromatography is introduced. In the first stage a multicomponent mixture is fractionated into three groups of chromatographic zones (fractions) by TLC where the adsorbent is deposited on an aluminum foil. At the next stage, the TLC plate is dried and cut by scissors into three relatively narrow strips containing the fractions separated. At the next stages each of these strips (“daughter” plates) containing the isolated group (fractions) is separated into individual components by an appropriate mobile phase using a solvent flow perpendicular to the flow of the mobile phase in the first stage. An example of the separation of a mixture of dyes illustrates the advantages of the method in practice. This method can be expanded for separation of mixtures, containing four or more fractions. This four-dimensional version of multidimensional TLC was carried out on plates with both open (conventional) and closed adsorbent layer.


Thin-layer chromatography Multi-dimensional planar chromatography Thin-layer chromatography with a closed adsorbent layer Thin-layer chromatography with an open adsorbent layer 


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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Victor Berezkin
    • 1
  • Svetlana Khrebtova
    • 1
  • Natalia Kulakova
    • 1
  1. 1.Topchiev Institute of Petrochemical SynthesisRussian Academy of SciencesMoscowRussia

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