Chromatographia

, Volume 71, Issue 9–10, pp 947–951 | Cite as

Simultaneous Determination of Four Major Conjugated Cholic Acids in Dry Bile of Sus scrofa domestica Brisson by MEKC

  • Jiao He
  • Jing Li
  • Yongmin Zhang
  • Yanguang He
  • Wenji Sun
Full Short Communication

Abstract

A simple, rapid and reproducible micellar electrokinetic chromatography method has been established for the simultaneous determination of four conjugated cholic acids, glycohyodeoxycholic acid, taurohyodeoxycholic acid, glycohyocholic acid and taurochenodeoxycholic acid, in dry bile of Sus scrofa domestica Brisson (Pig Gall Powder). The optimum separation conditions included 15 mM sodium tetraborate buffer solution containing 30 mM SDS, 10 mM β-CD, and 20% (v/v) acetonitrile (pH 10.5). Applied voltage was 20 kV and temperature was at 25 °C. Gentiopicroside was used as an internal standard and detection was at 200 nm. The effects on separation of buffer pH, buffer concentration, and organic modifiers were investigated. The extracts of dry bile of Sus scrofa domestica Brisson were well separated within 20 min.

Keywords

Micellar electrokinetic chromatography Conjugated cholic acid Sus scrofa domestica Brisson 

Notes

Acknowledgments

This work was financially supported by the Graduate Innovation and Creativity Funds of Northwest University, China (08YZZ36 and 09YZZ61).

References

  1. 1.
    Pharmacopoeia Committee (ed) (2005) Pharmacopoeia of the People’s Republic of China, vol 1. Chemical Industry Press, Beijing, p 222Google Scholar
  2. 2.
    Song LR (2001) Dictionary of modern Chinese medicine, vol 2. People’s Medical Publishing House Press, Beijing, p 1991Google Scholar
  3. 3.
    Li PF, Ling HX, Hong G (2008) Chin J Vet Sci 28:1317–1320Google Scholar
  4. 4.
    Ren YF, Lu YQ, Li XS, Tang PX (1989) Chin J Pharmacol Toxicol 3:236–240Google Scholar
  5. 5.
    Sun DN, Zou L (2009) Chin J Obstet Gynecol Pediatr 5:35–37Google Scholar
  6. 6.
    Wang K, Zhang XL, Ge YL, Ju XL (2007) Chem World 48:278–282Google Scholar
  7. 7.
    Mei HS, Shi CZ, Wang KP (1999) Chin J Biochem Pharm 20:198–200Google Scholar
  8. 8.
    Morita JR (1971) Jpn Cent Rev Med 275:518Google Scholar
  9. 9.
    Ding L, Zhang XX, Chang WB, Lin W, Yang M (2005) Chromatographia 61:85CrossRefGoogle Scholar
  10. 10.
    Ramautar R, Somsen GW, Jong de GJ (2007) Anal Bioanal Chem 387:293CrossRefGoogle Scholar
  11. 11.
    Rodríguez Flores J, Berzas JJ, Castañeda G, Rodríguez N (2003) J Chromatogr B 794:381–388CrossRefGoogle Scholar
  12. 12.
    Kowalski P (2008) J Pharm Biomed Anal 47:487–493CrossRefGoogle Scholar
  13. 13.
    Baker JS, Colón LA (2009) Chromatogr A 1216:9048–9054CrossRefGoogle Scholar
  14. 14.
    Gallardo A, Fernández F, Bermejo P, Rebuelta M, Cifuentes A, Díez-Masa J-C, Román JS (2000) Biomaterials 21:915–921CrossRefGoogle Scholar
  15. 15.
    Gomes P, Sippel J, Jablonski A, Steppe M (2004) J Pharm Biomed Anal 36:909–913CrossRefGoogle Scholar
  16. 16.
    Suntornsuk L, Pipitharome O, Wilairat P (2003) J Pharm Biomed Anal 33:441–449CrossRefGoogle Scholar
  17. 17.
    Hu Z, He LC, Zhang J, Luo GA (2006) J Chromatogr B 837:11–17CrossRefGoogle Scholar
  18. 18.
    Rodriguez VG, Lucangioli SE, Fernandez-Otero GC, Carducci CN (2000) J Pharm Biomed Anal 23:375–381CrossRefGoogle Scholar
  19. 19.
    Pharmacopoeial Convention (1995) United States Pharmacopoeia 23 and The National Formulary XVIII, second supplement. Rand McNally, Tauton, p 2688Google Scholar
  20. 20.
    Christopher Ehlen J, Elliott Albers H, Breyer ED (2005) J Neurosci Methods 147:36–47CrossRefGoogle Scholar
  21. 21.
    Zhao YF, Luo XP, Ming YF, Chen LR, Li YM (2006) J Pharm Biomed Anal 41:124–128CrossRefGoogle Scholar
  22. 22.
    Li J, Huang XY, Lai DW, Li J, Sun WJ (2008) Chromatographia 67:989–993CrossRefGoogle Scholar
  23. 23.
    Berzas JJ, Del Castillo B, Castañeda G, Pinilla MJ (1999) Talanta 50:261–268CrossRefGoogle Scholar
  24. 24.
    Zacharis CK, Tzanavaras PD, Notou M, Zotou A, Themelis DG (2009) J Pharm Biomed Anal 49:201–206CrossRefGoogle Scholar

Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Jiao He
    • 1
  • Jing Li
    • 1
  • Yongmin Zhang
    • 2
  • Yanguang He
    • 1
  • Wenji Sun
    • 1
  1. 1.Biomedicine Key Laboratory of Shaanxi ProvinceNorthwest UniversityXi’anPeople’s Republic of China
  2. 2.Institut Parisien de Chimie Moléculaire, Université Pierre and Marie Curie-Paris 6 (UMR CNRS 7201)ParisFrance

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