Abstract
A system enabling coupling of pressurized capillary electrochromatography (pCEC) with off-column amperometric detection (AD) is reported in which conduction of the current in pCEC was achieved through a cellulose acetate-coated porous polymer joint, and the effect of the high-voltage field applied to pCEC for AD was also eliminated. Effects of supplementary pressure on the porous polymer joint and the effects on AD of capillary columns of different i.d. were investigated. The performance of the pCEC–AD system with the porous polymer joint was evaluated with phenol and hydroquinone using sulfonated stearyl methacrylate monolithic columns. The separation efficiency of the column in pCEC–AD, using the proposed off-column detection with the cellulose acetate membrane joint, was comparable with that of pCEC–UV using on-column detection. Compared with end-column detection using a 50 μm i.d. capillary column without a joint, a higher signal-to-noise ratio was achieved, even using a 100 μm i.d. capillary column with a joint. Successful separation and detection of dopamine and epinephrine were also achieved by use of this system.
Similar content being viewed by others
References
Pretorius V, Hopkins BJ, Schieke JD (1974) J Chromatogr 99:23–30
Jorgenson JW, Lukacs KD (1981) J Chromatogr 218:209–216
Liu S, Xie Z, Wu X, Lin X, Guo L, Chen G (2005) J Chromatogr A 1092:258–262
Gfrörer P, Tseng L-H, Rapp E, Albert K, Bayer E (2001) Anal Chem 73:3234–3239
Ru QH, Luo GA, Fu YR (2001) J Chromatogr A 924:331–336
Yan C, Dadoo R, Zhao H, Zare RN, Rakestraw DJ (1995) Anal Chem 67:2026–2029
Lin Z, Xie Z, Lü H, Lin X, Wu X, Chen G (2006) Anal Chem 78:5322–5328
Lin Z, Wu X, Lin X, Xie Z (2007) J Chromatogr A 1170:118–121
Lin Z, Lin J, Wu X, Lin X, Xie Z (2008) Electrophoresis 29:401–409
Schmeer K, Behnke B, Bayer E (1995) Anal Chem 67:3656–3658
Pusecker K, Schewitz J, Gfrörer P, Tseng L-H, Albert K, Bayer E (1998) Anal Chem 70:3280–3285
Liang Z, Duan J, Zhang L, Zhang W, Zhang Y, Yan C (2004) Anal Chem 76:6935–6940
Gfrörer P, Tseng LH, Rapp E, Albert K, Bayer E (2001) Anal Chem 73:3234–3239
Pusecker K, Schewitz J, Gfrörer P, Tseng L, Albert K, Bayer E (1998) Anal Chem 70:3280–3285
Huang X, Zare RN, Sloss S, Ewing AG (1991) Anal Chem 63:189–192
Lu W, Cassidy RM (1994) Anal Chem 66:200–204
Liu S, Wu X, Xie Z, Lin X, Guo L, Yan C, Chen G (2005) Electrophoresis 26:2342–2350
Wallingford RA, Ewing AG (1987) Anal Chem 59:1762–1766
Yil YF, Lee HK, Li SFY, Khoo SB (1991) J Chromatogr 585:139–144
O’Shea TJ, Greenhagen RD, Lunte SM, Lunte CE, Smyth MR, Radzik DM, Watanabe NJ (1992) J Chromatogr 593:305–312
Park S, Lunte CE (1995) Anal Chem 67:4366–4370
Hu S, Wang ZL, Li PB, Cheng JK (1997) Anal Chem 69:264–267
Yin XB, Qiu H, Sun X, Yan J, Liu J, Wang E (2004) Anal Chem 76:3846–3850
Osbourn DM, Lunte CE (2001) Anal Chem 73:5961–5964
Whang CW, Chen IC (1992) Anal Chem 64:2461–2464
Fermier AM, Gostkowski ML, Colón LA (1996) Anal Chem 68:1661–1664
Bedair M, Rassi ZE (2002) Electrophoresis 23:2938–2948
Bedair M, Rassi ZE (2003) J Chromatogr A 1013:35–45
Acknowledgments
This work was supported by funding from the National High-Tech R&D Program (2006AA09Z161), the National Natural Science Foundation of China (20907009, 40976071), the Science Foundation of Fujian Province (2007J0129), the Key Science and Technology Project of Fujian Province (2007Y0062, 2007Y0022, 2008Y2004), the Science and Technology Project of Fuzhou University (2008-XQ-06), and the fund of Key Laboratory of Chinese Pharmacies for Fujian University (CKJ2008027).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, S., Lin, X., Zhang, X. et al. Off-Column Amperometric Detection for Pressurized Capillary Electrochromatography. Chroma 71, 659–665 (2010). https://doi.org/10.1365/s10337-010-1529-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-010-1529-6