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Chromatographia

, Volume 71, Issue 7–8, pp 727–732 | Cite as

Simultaneous Determination of Free and Esterified Fatty Alcohols, Phytosterols and Solanesol in Tobacco Leaves by GC

  • Yuxin Liu
  • Guoping Yong
  • Yingbo Xu
  • Dongliang Zhu
  • Hongwu Tong
  • Shaomin Liu
Full Short Communication

Abstract

A convenient gas chromatographic method equipped with flame ionization detection has been achieved for simultaneously determining six free and esterified fatty alcohols, three phytosterols, and solanesol in tobacco leaves. This analytic method consisted of direct alkaline hydrolysis, ultrasonic-assisted extraction and derivatization to trimethylsilyl ether derivatives. These three procedures did not require complicated and laborious preliminary purification steps of samples prior to GC, and the method was simple and convenient. The recovery of all target compounds was in the range of 85–105%, the relative standard deviations (RSDs) were less than 4%, and limits of detection (LOD) varied from 0.1–0.4 μg mL−1. The optimized methodology was successfully applied to determine free and esterified fatty alcohols, phytosterols and solanesol contents of different type tobacco samples.

Keywords

Gas chromatography Fatty alcohols Phytosterols and solanesol Tobacco leaves 

Notes

Acknowledgments

The author gratefully acknowledges the support of the technology center of China Tobacco Anhui Industrial Corporation.

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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Yuxin Liu
    • 1
    • 2
  • Guoping Yong
    • 2
  • Yingbo Xu
    • 3
  • Dongliang Zhu
    • 3
  • Hongwu Tong
    • 1
  • Shaomin Liu
    • 1
  1. 1.Research Center of Tobacco and HealthUniversity of Science and Technology of ChinaHefeiChina
  2. 2.Department of ChemistryUniversity of Science and Technology of ChinaHefeiChina
  3. 3.Technology Center of China Tobacco Anhui Industrial CorporationHefeiChina

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