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LC Determination of Tramadol, M1, M2, M4, and M5 in Plasma

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Abstract

A new LC procedure for the determination of tramadol, an analgesic, and its metabolites has been developed and validated. Following a liquid–liquid extraction using ethyl acetate and hexane, samples were separated by RP-LC on a Symmetry C18 column and quantified using fluorescence detection at an excitation of 202 nm and an emission of 296 nm. The mobile phase was a mixture of potassium dihydrogen phosphate buffer (0.01 M), 0.1% triethylamine (pH 2.9) and acetonitrile, with a flow-rate of 1.1 mL min−1. The standard curve ranged from 5 to 5,000 ng mL−1. Intra-and inter-assay variability for all the compounds were less that 10% and the average recovery was greater than 90%. This assay is suitable for use in pharmacokinetic studies.

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Authors and Affiliations

  1. Department of Comparative Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN, 37996, USA

    Jason Yarbrough, Marcy Souza & Sherry Cox

  2. Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 2407 River Drive, Knoxville, TN, 37996, USA

    Cheryl Greenacre

Authors
  1. Jason Yarbrough
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  2. Cheryl Greenacre
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  3. Marcy Souza
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  4. Sherry Cox
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Correspondence to Jason Yarbrough.

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Yarbrough, J., Greenacre, C., Souza, M. et al. LC Determination of Tramadol, M1, M2, M4, and M5 in Plasma. Chroma 71, 523–527 (2010). https://doi.org/10.1365/s10337-009-1451-y

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  • DOI: https://doi.org/10.1365/s10337-009-1451-y

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