Chromatographia

, Volume 71, Issue 1–2, pp 79–84 | Cite as

Retention Behaviour of Bupropion Hydrochloride in Reversed Phase Ion Pair LC and Validated Analysis of the Drug in Pharmaceuticals

Full Short Communication

Abstract

The chromatographic behaviour of bupropion hydrochloride, a basic drug of pK a 7.9, has been investigated under reversed-phase ion-pairing conditions and the results were used to develop a method for analysis of bupropion hydrochloride in pharmaceuticals. Chromatographic separation of bupropion hydrochloride and carbamazepine (used as internal standard) was performed on a C8 column (150 mm × 4.6 mm i.d., 3.5-μm particle), with 40:10:50 (v/v) methanol–acetonitrile–phosphate buffer (20 mm, pH 3.0), containing 10 mm 1-heptane sulfonic acid sodium salt (1-HSA), as optimum mobile phase at a flow rate of 1.0 mL min−1. UV detection was at 254 nm. The fully validated method enables reproducible and selective analysis of bupropion hydrochloride in pharmaceuticals.

Keywords

Column liquid chromatography Ion-pairing Pharmaceutical analysis Method validation Bupropion hydrochloride 

Notes

Acknowledgments

The authors are grateful to the Research Council of Anadolu University for support of this project (project no: 060324), to GlaxoSmithKline (Istanbul, Turkey) for the gift sample of BUP standard, and to Professor Dr Muzaffer Tuncel for his valuable help in all the authors’ studies. DY acknowledges a scholarship for PhD students from the Scientific and Technical Research Council of Turkey (TUBITAK).

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  1. 1.Department of Analytical Chemistry, Faculty of PharmacyAnadolu UniversityEskisehirTurkey

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