Chromatographia

, 70:1531 | Cite as

Synthesis of Molecularly Imprinted Polymers for Nevirapine by Dummy Template Imprinting Approach

  • Weeranuch Kareuhanon
  • Vannajan Sanghiran Lee
  • Piyarat Nimmanpipug
  • Chatchai Tayapiwatana
  • Mookda Pattarawarapan
Original

Abstract

Nevirapine (NVP) and its structurally related analogs including nicotinamide (NAM), benzamide (BZM) and benzophenone (BZP) were used as templates in the synthesis of molecularly imprinted polymers for NVP. Molecular modeling was used to estimate binding energy of the complex formation between methacrylic acid (MAA) monomer and the selected templates, while equilibrium binding studies were applied to evaluate the polymer binding efficiency. The data indicated that NAM is the best candidate to prepare MIPs for retaining NVP due to a relatively similar binding energy between the NVP–MAA and NAM–MAA complex. The NAM-imprinted polymer showed a high binding affinity and selectivity toward NVP. When the polymer was applied as a sorbent in solid-phase extraction of NVP from human plasma, high recovery and reproducibility were obtained.

Keywords

Column liquid chromatography Molecular imprinting and modeling Dummy template Nevirapine and nicotinamide 

Notes

Acknowledgments

The authors gratefully acknowledge the Government Pharmaceutical Organization (GPO), Thailand for providing NVP for this study, the Center of Excellence for Innovation in Chemistry (PERCH-CIC), the Thailand Research Fund (TRF) and Commission on Higher Education, Ministry of Education for financial support to this research.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Weeranuch Kareuhanon
    • 1
  • Vannajan Sanghiran Lee
    • 1
  • Piyarat Nimmanpipug
    • 1
  • Chatchai Tayapiwatana
    • 2
  • Mookda Pattarawarapan
    • 1
  1. 1.Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of ScienceChiang Mai UniversityChiang MaiThailand
  2. 2.Division of Clinical Immunology, Faculty of Associated Medical SciencesChiang Mai UniversityChiang MaiThailand

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