Synthesis of Molecularly Imprinted Polymers for Nevirapine by Dummy Template Imprinting Approach
Nevirapine (NVP) and its structurally related analogs including nicotinamide (NAM), benzamide (BZM) and benzophenone (BZP) were used as templates in the synthesis of molecularly imprinted polymers for NVP. Molecular modeling was used to estimate binding energy of the complex formation between methacrylic acid (MAA) monomer and the selected templates, while equilibrium binding studies were applied to evaluate the polymer binding efficiency. The data indicated that NAM is the best candidate to prepare MIPs for retaining NVP due to a relatively similar binding energy between the NVP–MAA and NAM–MAA complex. The NAM-imprinted polymer showed a high binding affinity and selectivity toward NVP. When the polymer was applied as a sorbent in solid-phase extraction of NVP from human plasma, high recovery and reproducibility were obtained.
KeywordsColumn liquid chromatography Molecular imprinting and modeling Dummy template Nevirapine and nicotinamide
The authors gratefully acknowledge the Government Pharmaceutical Organization (GPO), Thailand for providing NVP for this study, the Center of Excellence for Innovation in Chemistry (PERCH-CIC), the Thailand Research Fund (TRF) and Commission on Higher Education, Ministry of Education for financial support to this research.
- 8.Nicholls IA (1998) J Mol Recognit 11:79–82. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<79:AID-JMR394>3.0.CO;2-B CrossRefGoogle Scholar
- 19.Frisch MJ, Trucks GW, Schlegel HB, Gill PMW, Johnson BG, Robb MA et al (1995) Gaussian 94, Revision B.3ÈE.2. Gaussian, Inc., PittsburghGoogle Scholar
- 30.Gregg SJ, Sing KSW (1982) In: Adsorption, Surface Area and Porosity, 2nd edn. Academic Press, LondonGoogle Scholar