Chromatographia

, 70:1665 | Cite as

HPTLC Densitometric Quantification of Glycyrrhizin, Glycyrrhetinic Acid, Apigenin, Kaempferol and Quercetin from Glycyrrhiza glabra

  • Baljinder Singh
  • Pankaj Mungara
  • Manish Nivsarkar
  • Sheetal Anandjiwala
Original

Abstract

Glycyrrhiza glabra Linn., commonly known as liquorice, is a reputed drug of Ayurveda. In the present work we developed and validated densitometric methods for quantification of glycyrrhizin, glycyrrhetinic acid, apigenin, kaempferol and quercetin using HPTLC. The developed methods were found to be precise and accurate. The amount of glycyrrhizin, glycyrrhetinic acid, and quercetin was found to be 1.070, 0.84 and 0.271% w/w, respectively. Apigenin and kaempferol were quantified in free (0.007 and 0.033% w/w) as well as bound (0.021 and 0.074% w/w) forms. This is the first report of simultaneous quantification of glycyrrhetinic acid and apigenin as well as kaempferol and quercetin from G. glabra. Furthermore, no TLC densitometric methods have been reported for the quantification of apigenin, kaempferol and quercetin from G. glabra.

Keywords

Thin layer chromatography TLC densitometric methods Glycyrrhizin, glycyrrhetinic acid, apigenin, kaempferol and quercetin Glycyrrhiza glabra 

Notes

Acknowledgment

The authors are thankful to Prof. Harish Padh, Project Director, NIPER-Ahmedabad for providing the infrastructure and facilities. The authors are also thankful to the two anonymous reviewers for their valuable comments, suggestions and critical review of the manuscript.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Baljinder Singh
    • 1
  • Pankaj Mungara
    • 1
  • Manish Nivsarkar
    • 2
  • Sheetal Anandjiwala
    • 1
  1. 1.Department of Natural ProductsNational Institute of Pharmaceutical Education and ResearchAhmedabadIndia
  2. 2.Department of Pharmacology and ToxicologyB. V. Patel PERD CentreAhmedabadIndia

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