, Volume 70, Issue 9–10, pp 1515–1517 | Cite as

Simultaneous LC Analysis of Aloe-Emodin, Rhein, Emodin, and Chrysophanol in Rhizoma Rhei-Type Preparations

Limited Short Communication


A simple and sensitive liquid chromatographic method has been established for simultaneous analysis of four compounds (aloe-emodin, rhein, emodin, and chrysophanol) in Rhizoma Rhei-type preparations. The compounds were separated in less than 20 min on a C18 column with 70:30 methanol–0.2% aqueous acetic acid as mobile phase at a flow rate of 1 mL min−1. The method was validated for specificity, accuracy, precision, and limits of detection. Good linear regression data (r 2 > 0.9980) were obtained for all the calibration plots within the ranges tested. The method is an attractive alternative for evaluation of the quality of Rhizoma Rhei-type preparations.


Column liquid chromatography Traditional Chinese medicine Rhizoma Rhei Anthraquinones 



This project was supported by the National Natural Science Foundation of China (nos. 30572339 and 90409010).


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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  1. 1.Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya HospitalCentral South UniversityChangshaChina
  2. 2.Key Unit of Traditional Chinese Medicine Gan of SATCM, Xiangya HospitalCentral South UniversityChangshaChina
  3. 3.Key Unit of the 11th Five-year Plan of SATCM in Cerebrosis, Xiangya HospitalCentral South UniversityChangshaChina
  4. 4.Laboratory of Ethnopharmacology, West China HospitalSichuan UniversityChengduChina

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