Advertisement

Chromatographia

, 70:1461 | Cite as

Determination of Picoxystrobin and Pyraclostrobin by MEKC with On-Line Analyte Concentration

  • Cabrini F. de Souza
  • Alessandra L. M. C. da Cunha
  • Ricardo Queiroz Aucélio
Full Short Communication

Abstract

Micellar electrokinetic capillary chromatography was used for the determination of picoxystrobin and pyraclostrobin. The background electrolyte consisted of borate buffer (40 mmol L−1 pH 8.5), SDS (30 mmol L−1) and acetonitrile (15% in volume). Runs were made at 25 °C with 25 kV applied potential. The developed method was applied to analyte fortified urine samples. On-line analyte concentration, combined with a capillary of a longer optical path length, allowed limits of quantification of 8.6 × 10−8 mol L−1 for picoxystrobin and 1.8 × 10−7 mol L−1 for pyraclostrobin.

Keywords

Micellar electrokinetic capillary chromatography On-line analyte concentration Picoxystrobin Pyraclostrobin 

Notes

Acknowledgments

Authors thank FINEP, CNPq and FAPERJ for research grants and scholarships. Statsoft-Brazil is also acknowledged.

References

  1. 1.
    Santino A, Bolzoni L, Bandini M (2004) J Chromatogr A 1036:161–169CrossRefGoogle Scholar
  2. 2.
    Balba H (2007) J Environ Sci Heal B 42:441–451CrossRefGoogle Scholar
  3. 3.
    Abreu SM, Caboni P, Cabras P, Garau VL, Alves A (2006) Anal Chim Acta 573:291–297CrossRefGoogle Scholar
  4. 4.
    Agência Nacional de Vigilância Sanitária—ANVISA-Brazil, Índice Monográfico, P46 and P50Google Scholar
  5. 5.
    Pyell U (2006) Electrokinetic chromatography: theory, instrumentation and applications, 1t edn. Wiley, LondonGoogle Scholar
  6. 6.
    Garcia AJ, Font G, Pico Y (2007) J Chromatogr A 1153:104–113CrossRefGoogle Scholar
  7. 7.
    Hernandez BJ, Cifuentes A, Garcia MFJ, Rodriguez DMA (2005) Electrophoresis 26:980–989CrossRefGoogle Scholar
  8. 8.
    Hernandez BJ, Cifuentes A, Garcia MFJ, Rodriguez DMA (2005) J Sep Sci 28:948–956CrossRefGoogle Scholar
  9. 9.
    Ravelo-Perez LM, Borges JH, Cifuentes A, Delgado MAR (2007) Electrophoresis 28:1805–1814CrossRefGoogle Scholar
  10. 10.
    Ravelo-Perez LM, Borges JH, Miquel TMB, Delgado MAR (2007) J Sep Sci 30:3240–3246CrossRefGoogle Scholar
  11. 11.
    Ravelo-Perez LM, Borges JH, Miquel TMB, Delgado MAR (2007) Electrophoresis 28:4072–4081CrossRefGoogle Scholar
  12. 12.
    Ravelo-Perez LM, Borges JH, Miquel TMB, Delgado MAR (2008) Food Chem 111:764–770CrossRefGoogle Scholar
  13. 13.
    Ravelo-Perez LM, Borges JH, Miquel TMB, Delgado MAR (2008) J Chromatogr A 1185:151–154CrossRefGoogle Scholar
  14. 14.
    Terabe S, Kim JB (2003) J Pharm Biomed Anal 30:1625–1643CrossRefGoogle Scholar
  15. 15.
    Turiel E, Fernandez P (2000) Analyst 125:1725–1731CrossRefGoogle Scholar
  16. 16.
    Burgi DS, Chien RL (1991) Anal Chem 63:2042–2047CrossRefGoogle Scholar
  17. 17.
    Quirino JP, Terabe S (1999) Anal Chem 71:1638–1644CrossRefGoogle Scholar
  18. 18.
    Quirino JP, Kim JB, Terabe S (2002) J Chromatogr A 965:357–373CrossRefGoogle Scholar
  19. 19.
    Long GL, Winefordner JD (1983) Anal Chem 55:712A–724ACrossRefGoogle Scholar
  20. 20.
    ISO 5725 (1994) Accuracy (trueness and precision) of measurement methods and results, part 2: basic method for determination of repeatability and reproducibility of a standard measurement methodGoogle Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Cabrini F. de Souza
    • 1
  • Alessandra L. M. C. da Cunha
    • 1
  • Ricardo Queiroz Aucélio
    • 1
  1. 1.Departamento de QuímicaPontifícia Universidade Católica do Rio de JaneiroRio de JaneiroBrazil

Personalised recommendations