Advertisement

Chromatographia

, 70:1291 | Cite as

Enantioseparation of Dioxopromethazine Hydrochloride in Urine with Liquid–Liquid Extraction by CE-ECL Detection

  • Xia Li
  • Bo Song
  • Baiqing Yuan
  • Jinying Sun
  • Tianyan You
Limited Short Communication

Abstract

Capillary electrophoresis coupled with electrochemiluminescence detection was developed for the separation and determination of dioxopromethazine hydrochloride (DPZ) enantiomers. Performance parameters of the proposed method were evaluated. An improved separation of DPZ enantiomers could be achieved after adding boric acid to buffer. The enantiomers were completely separated with running buffer of 16.5 mM β-CD in 25 mM tris-H3PO4–40 mM H3BO3 at pH 2.5. The proposed method was successfully applied to the separation and determination of DPZ enantiomers in human urine with a liquid–liquid extraction procedure.

Keywords

Capillary electrophoresis-electrochemiluminescence detection Liquid–liquid extraction Enantioseparation Dioxopromethazine hydrochloride 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 20605020).

References

  1. 1.
    Chang HM, Tsai CF, Li CF (1998) J Agric Food Chem 46:4598–4603. doi: 10.1021/jf980157z CrossRefGoogle Scholar
  2. 2.
    Vespalec R, Boček P (2002) Chem Rev 100:3715–3753. doi: 10.1021/cr9411583 CrossRefGoogle Scholar
  3. 3.
    Chankvetadze B (2007) J Chromatogr A 1168:45–70. doi: 10.1016/j.chroma.2007.08.008 CrossRefGoogle Scholar
  4. 4.
    Kodama S, Ohura T, Yamamoto A, Matsunaga A, Kanbe T (2003) J Agric Food Chem 51:6128–6131. doi: 10.1021/jf030282x CrossRefGoogle Scholar
  5. 5.
    Deeb SE, Hasemann P, Wätzig H (2008) Electrophoresis 29:3552–3562. doi: 10.1002/elps.200800081 CrossRefGoogle Scholar
  6. 6.
    Li YH, Wang CY, Sun JY (2005) Anal Chim Acta 550:40–46. doi: 10.1016/j.aca.2005.06.045 CrossRefGoogle Scholar
  7. 7.
    Ren JC, Liu HW (1996) J Chromatogr A 732:175–181. doi: 10.1016/0021-9673(95)01250-8 CrossRefGoogle Scholar
  8. 8.
    Mikuš P, Valášková I, Havránek E (2003) J Pharm Biomed Anal 33:157–164. doi: 10.1016/S0731-7085(03)00254-1 CrossRefGoogle Scholar
  9. 9.
    Mikuš P, Valášková I, Havránek E (2005) J Pharm Biomed Anal 38:442–448. doi: 10.1016/j.jpba.2005.01.020 CrossRefGoogle Scholar
  10. 10.
    Mikuš P, Valášková I, Havránek E (2006) Talanta 70:840–846. doi: 10.1016/j.talanta.2006.02.013 CrossRefGoogle Scholar
  11. 11.
    Yin XB, Wang EK (2005) Anal Chim Acta 533:113–120. doi: 10.1016/j.aca.2004.11.015 CrossRefGoogle Scholar
  12. 12.
    Gerardi RD, Barnett NW, Lewis SW (1999) Anal Chim Acta 378:1–41. doi: 10.1016/S0003-2670(98)00545-5 CrossRefGoogle Scholar
  13. 13.
    Fang LY, Kang JZ, Yin XB, Yang XR, Wang EK (2006) Electrophoresis 27:4516–4522. doi: 10.1002/elps.200600114 CrossRefGoogle Scholar
  14. 14.
    Cao WD, Liu JF, Yang XR, Wang EK (2002) Electrophoresis 23:3683–3691. doi: 10.1002/1522-2683(200211)23:21<3683::AID-ELPS3683>3.0.CO;2-F CrossRefGoogle Scholar
  15. 15.
    Wren SAC, Rowe RC (1992) J Chromatogr 603:235–241. doi: 10.1016/0021-9673(92)85366-2 CrossRefGoogle Scholar
  16. 16.
    Sun JY, Xu XY, Wang CY, You TY (2008) Electrophoresis 29:3999–4007. doi: 10.1002/elps.200700875 CrossRefGoogle Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Xia Li
    • 1
    • 2
  • Bo Song
    • 2
  • Baiqing Yuan
    • 1
  • Jinying Sun
    • 1
  • Tianyan You
    • 1
  1. 1.State Key Laboratory of Electroanalytical ChemistryChangchun Institute of Applied Chemistry, Chinese Academy of SciencesChangchunChina
  2. 2.Changchun University of Science and TechnologyChangchunChina

Personalised recommendations