Rapid Separation of Hydrophobic Compounds by UPLC: in Case of Bisindolylmaleimides, Indolocarbazoles or Fullerene C60 Derivatives
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Rapid separation of hydrophobic compounds such as bisindolylmaleimides, indolocarbazoles or fullerene C60 derivatives by ultra performance liquid chromatography was developed. Bisindolylmaleimides and indolocarbazoles were separated within 7 and 5 min, respectively. The linear ranges for the determination of bisindolylmaleimides and indolocarbazoles were 1.25−500 pmol and 0.5−50 pmol per injection (r > 0.99), respectively. Fullerene C60 derivatives were separated within 9 min, and the linear range for the determination of fullerene C60 derivatives was 0.1−10 pmol per injection (r > 0.99).
KeywordsUltra performance liquid chromatography Bisindolylmaleimide Indolocarbazole Fullerene
We gratefully thank Waters (Tokyo, Japan) for the use of the Acquity UPLC system in the university program.