, Volume 70, Issue 5–6, pp 969–974 | Cite as

Quantitative Determination of Eight Major Constituents in the Traditional Chinese Medicinal Yi-Qi-Fu-Mai Preparation by LC

  • Dan-Dan Zhou
  • Shou-Jun Jiang
  • Ling Tong
  • Yue-Wu Yang
  • Gang-Li Wang
  • Zheng-Liang Ye
  • Zheng-Tao Wang
  • Rui-Chao Lin
Full Short Communication


A high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of eight marker components, ginsenoside Rf, Rb1, Rb2, Rb3, Rd, Rg3, 20(S)-ginsenoside F2 and schizandrin, present in traditional Chinese medicinal Yi-Qi-Fu-Mai preparations. Samples were prepared using a solid phase extraction procedure. Chromatographic separation was carried out on a Waters Symmetry C18 column (4.6 × 250 mm, 5 μm) by stepwise gradient elution with water (0.05% phosphoric acid, v/v) and acetonitrile as the mobile phase. Good linear relationships between were observed between peak areas and concentrations with r 2 values above 0.9990 for all the analytes. Average recoveries for the eight markers ranged from 97.7 to 100.4%. This method was found to be rapid, sensitive, accurate, and could be readily applied to the quality assessment of Yi-Qi-Fu-Mai preparations.


Column liquid chromatography Ginsenosides Schizandrin Yi-Qi-Fu-Mai Quality control 


  1. 1.
    WHO/EDM/TRM (2002) WHO traditional medicine strategy 2002–2005. World Health Organization, GenevaGoogle Scholar
  2. 2.
    Luo GA, Wang YM, Cao J, Yang XD (2004) World Sci Tech Modern Trad Chin Med 4:5–12Google Scholar
  3. 3.
    Cheng YY, Chen MJ (2003) Chem J Inf Comput Sci 43:1068–1070Google Scholar
  4. 4.
    Pharmacopoeia Committee of People’s Republic of China (2005) Chinese Pharmacopoeia. Chemical Industry Press, BeijingGoogle Scholar
  5. 5.
    Cicero AF, Vitale G, Savino G, Arletti R (2003) Phytother Res 17:174–178. doi: 10.1002/ptr.1262 CrossRefGoogle Scholar
  6. 6.
    Shin YW, Bae EA, Kim DH (2006) Arch Pharm Res 29:685–690. doi: 10.1007/BF02968253 CrossRefGoogle Scholar
  7. 7.
    Yue PY, Wong DY, Wu PK, Leung PY (2006) Biochem Pharmacol 72:437–445. doi: 10.1016/j.bcp.2006.04.034 CrossRefGoogle Scholar
  8. 8.
    Hancke JL, Brugos RA, Ahumada F (1999) Fitoterapia 70:451–471. doi: 10.1016/S0367-326X(99)00102-1 CrossRefGoogle Scholar
  9. 9.
    He XG, Lian LZ, Lin LZ (1997) J Chromatogr A 757:81–87. doi: 10.1016/S0021-9673(96)00685-1 CrossRefGoogle Scholar
  10. 10.
    Wan JB, Li SP, Rong JJ, Yi KL, Wang YT (2004) Chin J Nat Med 2:215–218Google Scholar
  11. 11.
    Zhang X, Zhu TQ (2001) China J Chin Mater Med 26:54Google Scholar
  12. 12.
    Lau AJ, Seo BH, Woo SO, Koh HL (2004) J Chromatogr A 1057:141–149. doi: 10.1016/j.chroma.2004.09.069 CrossRefGoogle Scholar
  13. 13.
    Li L, Zhang JL, Sheng YX, Guo DA, Wang Q, Guo HZ (2005) J Pharm Biomed Anal 38:45–51. doi: 10.1016/j.jpba.2005.01.014 CrossRefGoogle Scholar
  14. 14.
    Wan JB, Lai CM, Li SP, Lee MY, Wang YT (2006) J Pharm Biomed Anal 41:274–279. doi: 10.1016/j.jpba.2005.10.023 CrossRefGoogle Scholar
  15. 15.
    Li L, Sheng Y, Zhang J, Guo D (2005) J Chromatogr Sci 43:421–425Google Scholar
  16. 16.
    Wan JB, Yang FQ, Li SP, Wang YT, Cui XM (2006) J Pharm Biomed Anal 41:1596–1601. doi: 10.1016/j.jpba.2006.01.058 CrossRefGoogle Scholar
  17. 17.
    Wan JB, Li P, Li SP, Wang YT, Dong TT-X, Tsim KW-K (2006) J Sep Sci 29:2190–2196. doi: 10.1002/jssc.200600103 CrossRefGoogle Scholar
  18. 18.
    Kim SN, Ha YW, Shin H, Son SH, Wu SJ, Kim YS (2007) J Pharm Biomed Anal 45:164–170. doi: 10.1016/j.jpba.2007.05.001 CrossRefGoogle Scholar
  19. 19.
    Li L, Tsao R, Dou JP, Song FR (2005) Anal Chim Acta 536:21–28. doi: 10.1016/j.aca.2004.12.064 CrossRefGoogle Scholar
  20. 20.
    Lai CM, Li SP, Yu H, Wan JB, Kan KW, Wang YT (2006) J Pharm Biomed Anal 40:669–678. doi: 10.1016/j.jpba.2005.11.003 CrossRefGoogle Scholar
  21. 21.
    Gu Y, Wang GJ, Sun JG, Jia YW, Xie HT, Wang W (2006) Anal Bioanal Chem 386:2043–2053. doi: 10.1007/s00216-006-0857-8 CrossRefGoogle Scholar
  22. 22.
    Ji QC, Harkey MR, Gershwin ME, Stern JS, Hackman RM (2001) Phytochem Anal 12:320–326. doi: 10.1002/pca.593 CrossRefGoogle Scholar
  23. 23.
    Xu MJ, Wang GJ, Xie HT, Huang Q, Wang W, Jia YW (2008) J Ethnopharmacol 115:483–488. doi: 10.1016/j.jep.2007.10.016 CrossRefGoogle Scholar
  24. 24.
    Guan J, Lai CM, Li SP (2007) J Pharm Biomed Anal 44:996–1000. doi: 10.1016/j.jpba.2007.03.032 CrossRefGoogle Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Dan-Dan Zhou
    • 1
    • 2
  • Shou-Jun Jiang
    • 2
  • Ling Tong
    • 3
  • Yue-Wu Yang
    • 4
  • Gang-Li Wang
    • 2
  • Zheng-Liang Ye
    • 4
  • Zheng-Tao Wang
    • 1
  • Rui-Chao Lin
    • 2
  1. 1.Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghaiPeople’s Republic of China
  2. 2.National Institute for the Control of Pharmaceutical and Biomedical ProductsBeijingPeople’s Republic of China
  3. 3.Shenyang Pharmaceutical UniversityShenyangPeople’s Republic of China
  4. 4.Tianjin Tasly Pride Pharmaceutical Company LimitedTianjinPeople’s Republic of China

Personalised recommendations