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Simultaneous Chromatographic Fingerprinting and Quantitative Analysis of Flemingia philippinensis by LC–DAD

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Abstract

To evaluate the quality of Flemingia philippinensis, a validated reversed-phase high-performance liquid chromatographic method with diode-array detection has been developed for chromatographic fingerprint analysis and for quantification of genistin, genistein, and 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone, the three main bioactive isoflavones. In the fingerprint analysis, 21 chromatographic peaks were selected as characteristic peaks; among these 14 flavonoids were identified on the basis of their retention times and UV spectra. In quantitative analysis, the recovery of the method was in the range 94.5–107.7%, and good linearity (r 2 > 0.9990) was observed for all the compounds over a relatively wide range of concentrations. The relative standard deviation of the method was less than 2.5% for intra-day and inter-day assays. The assay was successfully applied to analysis of the three active isoflavones in 19 samples. The results indicated the assay is reproducible and precise and could be used for convenient assessment of the quality of F. philippinensis.

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Acknowledgment

This research was financially supported by a grant from the Project on Tackling Key Problems in Science and Technology in Guangxi, China (no. 0718009-2C). The authors wish to thank Mr. Lijun Shi, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, P. R. China, for collecting samples.

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, People’s Republic of China

    Hua Li, Meihua Yang & Xiaojun Ma

  2. College of Food Science and Technology, Henan University of Technology, 450052, Zhengzhou, People’s Republic of China

    Hua Li

  3. Guangxi Botanical Garden of Medicinal Plant, 530023, Nanning, People’s Republic of China

    Jianhua Miao & Xiaojun Ma

Authors
  1. Hua Li
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  2. Meihua Yang
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  3. Jianhua Miao
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  4. Xiaojun Ma
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Correspondence to Xiaojun Ma.

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Li, H., Yang, M., Miao, J. et al. Simultaneous Chromatographic Fingerprinting and Quantitative Analysis of Flemingia philippinensis by LC–DAD. Chroma 70, 447–454 (2009). https://doi.org/10.1365/s10337-009-1238-1

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