Skip to main content
SpringerLink
Log in

Determination and Pharmacokinetics of Isoliquiritigenin in Rat Plasma by RP–LC After Intravenous Administration

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A simple, rapid and sensitive reverse phase liquid chromatography-diode array detector method has been developed and validated for the determination of isoliquiritigenin in rat plasma using acetanilide as an internal standard. The plasma was deproteinized with acetonitrile and separated from the aqueous layer by adding sodium chloride. The mobile phase was acetonitrile, 0.05 M potassium dihydrogen phosphate and triethylamine (50:50:0.5, v/v/v) (pH 2.00). Detection wavelength was set at 242 nm during 0–5 min and 362 nm during 5–9 min. The limit of quantification was 0.019 μg mL−1. The mean accuracy was 96.851–98.140%. Extract recoveries at concentration of 0.038, 0.625, 1.250, 5.000 and 20.000 μg mL−1 were 82.740, 80.814, 80.920, 80.978 and 81.103%, respectively. The validated method was successfully applied to the pharmacokinetic study of ISL in rat plasma after intravenous administration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Yen KY (1996) An illustrated pharmacognosy. SMC Publishing Inc, Taipei, pp 165–172

    Google Scholar 

  2. Hayashi H, Hiraoka N, Ikeshiro Y, Yamamoto H (1996) Plant Sci 116:233–239. doi:10.1016/0168-9452(96)04387-7

    Article  CAS  Google Scholar 

  3. Hayashi H, Hiraoka N, Ikeshiro Y, Yamamoto H, Yoshikawa T (1998) Biol Pharm Bull 21:987–989

    CAS  Google Scholar 

  4. Kitagawa I, Chen WZ, Hori K, Harada E, Yasuda N, Yoshikawa M, Ren J (1994) Chem Pharm Bull (Tokyo) 42:1056–1057

    CAS  Google Scholar 

  5. Kitagawa I, Chen WZ, Hori K, Kobayashi M, Ren J (1998) Chem Pharm Bull (Tokyo) 46:1511–1513

    CAS  Google Scholar 

  6. Vaya J, Belinky PA, Aviram M (1997) Free Radic Biol Med 23:302–313. doi:10.1016/S0891-5849(97)00089-0

    Article  CAS  Google Scholar 

  7. Yu X, Wang W, Yang M (2007) Food Chem 104:715–720. doi:10.1016/j.foodchem.2006.10.081

    Article  CAS  Google Scholar 

  8. Morteza-Semnani K, Saeedi M, Hamidian M (2004) Fitoterapia 75:123–129. doi:10.1016/j.fitote.2003.12.007

    Article  CAS  Google Scholar 

  9. Chan SC, Chang YS, Wang JP, Chen SC, Kuo SC (1998) Planta Med 64:153–158. doi:10.1055/s-2006-957394

    Article  CAS  Google Scholar 

  10. Kim SC, Byun SH, Yang CH, Kim CY, Kim JW, Kim SG (2004) Toxicol 197:239–251. doi:10.1016/j.tox.2004.01.010

    Article  CAS  Google Scholar 

  11. Tawata M, Aida K, Noguchi T (1992) Eur J Pharmacol 212:87–92. doi:10.1016/0014-2999(92)90076-G

    Article  CAS  Google Scholar 

  12. Chisato N, Jing Y, Junko K, Masahiro M (2008) J Mol Cell Cardiol 44:444–461

    Google Scholar 

  13. Hsua Y-L, Kuob P-L, Lin C-C (2005) Life Sci 77:279–292. doi:10.1016/j.lfs.2004.09.047

    Article  CAS  Google Scholar 

  14. Kobayashi S, Miyamoto T, Kimura I, Kimura M (1995) Biol Pharm Bull 18:1382–1383

    CAS  Google Scholar 

  15. Baba M, Asano R, Takigami I, Takahashi T, Ohmura M, Okada Y, Sugimoto H, Arika T, Nishino H, Okuyama T (2002) Biol Pharm Bull 25:247–250. doi:10.1248/bpb.25.247

    Article  CAS  Google Scholar 

  16. Yamamoto S, Aizu E, Jiang H, Nakadate T, Kiyoto I, Wang JC, Kato R (1991) Carcino 12:317–323. doi:10.1093/carcin/12.2.317

    Article  CAS  Google Scholar 

  17. Yamazaki S, Morita T, Endo H, Hamamoto T, Baba M, Joichi Y, Kaneko S, Okada Y, Okuyama T, Nishino H, Tokue A (2002) Cancer Lett 183:23–30. doi:10.1016/S0304-3835(02)00113-1

    Article  CAS  Google Scholar 

  18. Kanazawa M, Satomi Y, Mizutani Y, Ukimura O, Kawauchi A, Sakai T, Baba M, Okuyama T, Nishino H, Miki T (2003) Eur Urol 43:580–586. doi:10.1016/S0302-2838(03)00090-3

    Article  CAS  Google Scholar 

  19. Maggiolini M, Statti G, Vivacqua A, Gabriele S, Rago V, Loizzo M, Menichini F, Amdo S (2002) J Steroid Biochem Mol Biol 82:315–322. doi:10.1016/S0960-0760(02)00230-3

    Article  CAS  Google Scholar 

  20. Ma J, Fu NY, Pang DB, Wu WY, Xu AL (2001) Planta Med 67:754–757. doi:10.1055/s-2001-18361

    Article  CAS  Google Scholar 

  21. Iwashita K, Kobori M, Yamaki K, Tsushida T (2000) Biosci Biotechnol Biochem 64:1813–1820. doi:10.1271/bbb.64.1813

    Article  CAS  Google Scholar 

  22. Jung JI, Lim SS, Choi HJ, Cho HJ, Shin H-K, Kim EJ, Chung W-Y, Park K-K, Jung HYP (2006) J Nutr Biochem 17:689–696. doi:10.1016/j.jnutbio.2005.11.006

    Article  CAS  Google Scholar 

  23. Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S (2003) J Agric Food Chem 51:1201–1210. doi:10.1021/jf020935u

    Article  CAS  Google Scholar 

  24. Takahashi T, Takasuka N, Iigo M, Baba M, Nishino H, Tsuda H (2004) Cancer Sci 95:448–453. doi:10.1111/j.1349-7006.2004.tb03230.x

    Article  CAS  Google Scholar 

  25. Zuo F, Zhou ZM, Liu ML (2001) Biol Pharm Bull 24:693–699. doi:10.1248/bpb.24.693

    Article  CAS  Google Scholar 

  26. Zuo F, Zhou ZM, Yan MZ, Liu ML, Xiong YL, Zhang Q, Song HY, Ye WH (2002) Biol Pharm Bull 25:558–567. doi:10.1248/bpb.25.558

    Article  CAS  Google Scholar 

  27. Li T, Wang T (1999) J Tradit Chin Med 24:44–50

    CAS  Google Scholar 

  28. Ma CJ, Li GS, Zhang DL, Liu K, Fan X (2005) J Chromatogr A 1078:188–197. doi:10.1016/j.chroma.2005.01.053

    Article  CAS  Google Scholar 

  29. Xie J, Wang W, Zhang Y, Bai Y, Yang Q (2007) J Pharm Biomed Anal 45:450–455. doi:10.1016/j.jpba.2007.07.011

    Article  CAS  Google Scholar 

  30. Wang Y-C, Yang Y-S (2007) J Chromatogr B Analyt Technol Biomed Life Sci 850:392–399. doi:10.1016/j.jchromb.2006.12.032

    Article  CAS  Google Scholar 

  31. Wicks DA, Li PCH (2004) Anal Chim Acta 507:107–114. doi:10.1016/j.aca.2003.09.067

    Google Scholar 

  32. Cao Y, Wang Y, Ji C, Ye J (2004) J Chromatogr A 1042:203–209. doi:10.1016/j.chroma.2004.05.049

    Article  CAS  Google Scholar 

  33. Li L, Liang S, Du F, Li C (2007) J Am Soc Mass Spectrom 18:778–787. doi:10.1016/j.jasms.2006.12.011

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Technological help from Ph.D. Yuming Dong (College of Pharmacy, Lanzhou University) and Ph.D. Wenli Zhang (College of Pharmacy, China Pharmaceutical University) is gratefully acknowledged.

Author information

Authors and Affiliations

  1. College of Pharmacy, China Pharmaceutical University, 210009, Nanjing, People’s Republic of China

    Xiaoyun Zhang & Jianping Liu

  2. College of Pharmacy, Lanzhou University, 730000, Gansu, People’s Republic of China

    Xiaoyun Zhang, Jingman Ni & Yanbin Shi

  3. Department of Pharmaceutics, The First Hospital of Lanzhou University, 730000, Gansu, People’s Republic of China

    Hua Qiao

Authors
  1. Xiaoyun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jianping Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hua Qiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jingman Ni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yanbin Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jianping Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, X., Liu, J., Qiao, H. et al. Determination and Pharmacokinetics of Isoliquiritigenin in Rat Plasma by RP–LC After Intravenous Administration. Chroma 70, 423–430 (2009). https://doi.org/10.1365/s10337-009-1236-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-009-1236-3

Keywords

Search

Navigation