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Purification Studies of Thymosin α-1 in LC

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Abstract

The screening in column liquid chromatography of several reversed phase silica gels with various mobile phases was studied for the purification of thymosin α-1. Loading studies on analytical columns were developed and transposed to the preparative scale with a thymosin α-1 injection concentration of 1 g L−1. To obtain thymosin α-1 with a purity higher than 98%, a local pre-concentration was applied at the head of the column.

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Authors and Affiliations

  1. Centre Interdisciplinaire de Nanosciences de Marseille (CNRS-UPR 3118) Aix-Marseille Université, Campus de luminy, Case 913, 13 288, Marseille cedex 9, France

    Guy Félix

  2. Kiralya, Parc Biocitech, Romainville, France

    Pascal Joly & Serge Droux

  3. Fuji Silysia Chemical S.A., En Budron, 1052, Le Mont-sur-Lausanne, Switzerland

    Lucien Charles

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  1. Guy Félix
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  2. Pascal Joly
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  3. Serge Droux
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  4. Lucien Charles
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Correspondence to Guy Félix.

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Félix, G., Joly, P., Droux, S. et al. Purification Studies of Thymosin α-1 in LC. Chroma 70, 381–387 (2009). https://doi.org/10.1365/s10337-009-1194-9

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  • DOI: https://doi.org/10.1365/s10337-009-1194-9

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