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An LC–MS–MS Method for the Simultaneous Quantitation of Acyclovir and Valacyclovir in Human Plasma

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Abstract

A simple, sensitive and selective LC–MS–MS method has been developed for the simultaneous determination of acyclovir and valacyclovir in human plasma. Acyclovir and valacyclovir in plasma were concentrated by solid phase extraction and chromatographed on a C18 column using a mobile phase of 0.1% formic acid: methanol (30:70% v/v). The method was validated over a linear range of 47–10,255 and 5–1,075 ng mL−1 for acyclovir and valacyclovir respectively. The LOQs were 47.6 and 5.0 ng mL−1. The validated method was applied for the quantitation of acyclovir and valacyclovir from plasma samples in a pharmacokinetic study.

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Acknowledgments

The authors wish to thank the following for their valuable support in shaping this manuscript, Prof. V. Venkateswaralu, Dr. Reddy’s Laboratories Limited, Dr. Shashank Lulay, Senior Director, Dr. Reddy’s Laboratories Limited and Mr. Indubhushan and Mr. C. V. Sathyendranath for their constant encouragement.

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Authors and Affiliations

  1. Department of Pharmacology, LM College of Pharmacy, Gujarat University, Ahmadabad, 380015, India

    Ravi Kanneti & Parloop A. Bhatt

  2. Department of Biopharmaceutics, Integrated Product Development, Dr. Reddy’s Laboratories Ltd, Hyderabad, 500072, India

    J. R. Aravinda Raj

  3. Clinical Pharmacology and Pharmacokinetics, Dr. Reddy’s Laboratories Limited, Hyderabad, 500072, India

    Ravi Kanneti & Ramadass Rajesh

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  1. Ravi Kanneti
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  2. Ramadass Rajesh
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Correspondence to Ravi Kanneti.

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Kanneti, R., Rajesh, R., Aravinda Raj, J.R. et al. An LC–MS–MS Method for the Simultaneous Quantitation of Acyclovir and Valacyclovir in Human Plasma. Chroma 70, 407–414 (2009). https://doi.org/10.1365/s10337-009-1171-3

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  • DOI: https://doi.org/10.1365/s10337-009-1171-3

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