Determination of Toosendanin Extracted from Traditional Chinese Medicine: the Fruit of Melia azedarach LC–FD Detection
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A rapid and selective reversed-phase liquid chromatography with fluorescence detection was developed and validated for the determination of toosendanin in the fruit of Melia azedarach. After extraction, the extracts were analysed at 25 °C using Eclipse XDB-C18 column (4.6 mm × 150 mm; 5 μm) and a mobile phase consisting of methanol–water at a flow rate of 1.0 mL min−1. Detection wavelength was set at λEx = 230 nm, λEm = 320 nm. The linearity, accuracy, and precision of the method were good, and the method can be successfully used to investigate the level of toosendanin component in herb samples with satisfactory results.
KeywordsColumn liquid chromatography Toosendanin Melia azedarach
The authors gratefully acknowledge the support by the project of the Chongqing Municipal Key Laboratory on Luminescence and Real-Time Analysis, Southwest University, China (CSTC, 2006CA8006).
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