Chromatographia

, Volume 70, Issue 3–4, pp 371–379 | Cite as

Optimization of Micellar LC Conditions for the Flavonoid Separation

  • Artem U. Kulikov
  • Marina N. Galat
  • Alexander P. Boichenko
Original

Abstract

The chromatographic conditions for the separation of a complex set of flavonoids (aglycones and glycosides) by micellar liquid chromatography with spectrophotometric detection were optimized. A good separation for all analytes was obtained and satisfactory peak shapes were achieved by isocratic elution with Ultrasphere ODS column (250 mm × 4.6 mm, 5 μm). The optimal mobile phase range for flavonoids separation is: SDS concentration between 0.014 and 0.018 mol L−1 and 1-propanol volume fraction between 2.2 and 4.5% (v/v) in a diluted (1:5) phosphate buffer solution pH 6.86. The flavonoids (robinin, rutin, hyperoside, quercitrin, liquroside, luteolin-7O-glucoside, apigenin-7O-glucoside, isosalipurposide, myricetin, fisetin, luteolin, apigenin, quercetin and caempferol) were successfully separated within 40 min with isocratic elution. The developed method is an alternative to reversed-phase LC in the assay of flavonoids in plants, plant extracts and plant extract containing drugs.

Keywords

Micellar liquid chromatography Isocratic elution Mobile phase optimization Flavonoids Apigenin-7O-glucoside Luteolin-7O-glucoside 

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Artem U. Kulikov
    • 1
    • 2
  • Marina N. Galat
    • 2
  • Alexander P. Boichenko
    • 2
  1. 1.Laboratory of Pharmacopoeial AnalysisScientific and Expert Pharmacopoeial CentreKharkovUkraine
  2. 2.Department of Chemical MetrologyKharkov V.N. Karazin National UniversityKharkovUkraine

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