Chromatographia

, Volume 70, Issue 3–4, pp 467–473 | Cite as

Determination of Aristolochic Acid I and II in Traditional Chinese Medicine by HPCE with Label Free Intrinsic Imaging

Original

Abstract

Aristolochic acids (AAs) I and II are the main bioactive ingredients in most Aristolochia plants, which are used for dietary supplements, slimming pills and Traditional Chinese Medicines (TCMs). Excessive ingestion of AAs can lead to serious nephropathy and has also been discovered to cause mutagenicity and carcinogenicity. Therefore, quantitative analysis and quality control for medicines containing AA’s are of great importance. The analysis of aristolochic acid I and II in TCM was achieved successfully in less than 5 min by capillary electrophoresis with label free intrinsic imaging (LFII) and UV detection at λ214 nm. The background electrolyte was deltaDOT’s TCM running buffer containing sodium tetraborate and β-cyclodextrin at pH 9.7. The results show very good resolution, precision and short analysis time with a detection limit of 1.23 × 10−7 and 8.83 × 10−8 mol L−1 for AAI and AAII, respectively. Comparative experiments were performed using conventional CE analysis with UV detection. The results emphasise the significant improvements achieved by the new technology. Label free intrinsic imaging is a powerful technology and can be used in the analytical arena in numerous applications including the analysis of the increasingly growing field of herbal medicine.

Keywords

High performance capillary electrophoresis Label free intrinsic imaging Aristolochic acid β-Cyclodextrin 

References

  1. 1.
    Lee TY, Wu ML, Deng JF, Hwang DF (2001) J Chromatogr B 766:169. doi:10.1016/S0378-4347(01)00416-9 Google Scholar
  2. 2.
    Vanherweghem JL (1998) J Altern Complem Med 4:9. doi:10.1089/acm.1998.4.1-9 CrossRefGoogle Scholar
  3. 3.
    Cosyns JP (2003) Drug Saf 26:33. doi:10.2165/00002018-200326010-00004 CrossRefGoogle Scholar
  4. 4.
    Fernando RC, Schmeiser HH, Scherf HR, Wiessler M (1993) IARC Sci Pub 124:167Google Scholar
  5. 5.
    Dong H, Suzuki N, Torres MC, Bonala RR, Johnson F, Grollman AP, Shibutani S (2006) Am Soc Pharmacol Exp Ther 34:1122Google Scholar
  6. 6.
    IARC Monogr Eval Carcinog Risks Hum (2002) 82:69Google Scholar
  7. 7.
    Wei L, Gong S, Wen D, Che B, Liao Y, Liu H, Feng X, Hu S (2004) J Chromatogr A 1049:211Google Scholar
  8. 8.
    Food and Drug Administration (FDA) dietary supplement: aristolochic acid, consumer advisory, April 2001. (http://www.cfsan.fda.gov/~dms/addsbot.html)
  9. 9.
    Li W, Li RK, Bo T, Liu HW, Feng XF, Hu SI (2004) Chromatographia 59:233. doi:10.1365/s10337-004-0314-9 CrossRefGoogle Scholar
  10. 10.
    Chan SA, Chen MJ, Liu TY, Fuh MR, Deng JF, Wu ML, Hsieh SJ (2003) Talanta 60:679. doi:10.1016/S0039-9140(03)00142-5 CrossRefGoogle Scholar
  11. 11.
    Lee MC, Tsao CH, Iou SC, Chuang WC, Sheu SJ (2003) J Sep Sci 26:818. doi:10.1002/jssc.200301271 CrossRefGoogle Scholar
  12. 12.
    Platter A, Reich E (2004) JPC-J Planar Chromatogr 17:355. doi:10.1556/JPC.17.2004.5.6 CrossRefGoogle Scholar
  13. 13.
    Platter A, Reich E (2004) J Liq Chromatogr RT 27:2087. doi:10.1081/JLC-120039420 CrossRefGoogle Scholar
  14. 14.
    Hashimoto K, Higuchi M, Makino B (1999) J Ethnopharmacol 64:185. doi:10.1016/S0378-8741(98)00123-8 CrossRefGoogle Scholar
  15. 15.
  16. 16.
    Ma XQ, Shi Q, Duan JA, Dong TTX, Tsim KWK (2002) J Agric Food Chem 50:486. doi:10.1021/jf0202279 CrossRefGoogle Scholar
  17. 17.
    Ku YR, Chang YS, Wen KC, Ho LK (1993) J Chromatogr A 848:537. doi:10.1016/S0021-9673(99)00475-6 CrossRefGoogle Scholar
  18. 18.
    Hsieh S-C, Huang M-F, Lin B-S, Chang H-T (2006) J Chromatogr A 1105:127. doi:10.1016/j.chroma.2005.07.056 CrossRefGoogle Scholar
  19. 19.
    Parlow JJ, Stevens AM, Stegeman RA, Stallings WC, Kurumbail RG, South MS (2003) J Med Chem 46:4297. doi:10.1021/jm030233b CrossRefGoogle Scholar
  20. 20.
    Gill AI, Frederickson M, Cleasby A, Woodhead SJ, Maria G, Carr MG, Woodhead AJ, Walker MT, Congreve MS, Devine LA, Tisi D, O’Reilly M, Seavers LCA, Davis DJ, Curry J, Antony R, Padova A, Murray CW, Carr RAE, Jhoti H (2005) J Med Chem 48:414. doi:10.1021/jm049575n CrossRefGoogle Scholar
  21. 21.
    Zhou X, Zheng C, Sun J, You T (2006) J Chromatogr A 1109:152CrossRefGoogle Scholar
  22. 22.
    Peng YY, Liu FH, Ye JN (2005) Electrophoresis 17:356Google Scholar
  23. 23.
    Zhou YX, Lei HM, Xu YH, Wei LX, Xu XF (2002) Research technology of fingerprint of Chinese traditional medicine. Press of Chemical Industry, BeijingGoogle Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  1. 1.Microseparation Group, Pharmaceutical Science Research DivisionKing’s College LondonLondonUK
  2. 2.deltaDOT LtdLondonUK

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