Determination of Aristolochic Acid I and II in Traditional Chinese Medicine by HPCE with Label Free Intrinsic Imaging
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- Dahab, A.A., Smith, N.W. & Marlin, N. Chroma (2009) 70: 467. doi:10.1365/s10337-009-1156-2
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Aristolochic acids (AAs) I and II are the main bioactive ingredients in most Aristolochia plants, which are used for dietary supplements, slimming pills and Traditional Chinese Medicines (TCMs). Excessive ingestion of AAs can lead to serious nephropathy and has also been discovered to cause mutagenicity and carcinogenicity. Therefore, quantitative analysis and quality control for medicines containing AA’s are of great importance. The analysis of aristolochic acid I and II in TCM was achieved successfully in less than 5 min by capillary electrophoresis with label free intrinsic imaging (LFII) and UV detection at λ214 nm. The background electrolyte was deltaDOT’s TCM running buffer containing sodium tetraborate and β-cyclodextrin at pH 9.7. The results show very good resolution, precision and short analysis time with a detection limit of 1.23 × 10−7 and 8.83 × 10−8 mol L−1 for AAI and AAII, respectively. Comparative experiments were performed using conventional CE analysis with UV detection. The results emphasise the significant improvements achieved by the new technology. Label free intrinsic imaging is a powerful technology and can be used in the analytical arena in numerous applications including the analysis of the increasingly growing field of herbal medicine.