, Volume 70, Issue 1–2, pp 299–303 | Cite as

Simultaneous Determination of Four Alkaloids in Gan-Yan-Ling Injection by GC-MS

  • Na Chen
  • Zheng Han
  • Lianjun Luan
  • Yongjiang Wu
Full Short Communication


A new separation and quantification method based on GC-MS in the selective ion mode for simultaneous determination of four alkaloids in Gan-Yan-Ling injection was developed. The calibration curves are linear over the range of 0.0125–0.555 mg mL−1 for cytisine, 0.009455–1.008 mg mL−1 for sophocarpine, 0.04739–1.516 mg mL−1 for matrine and 0.01135–1.211 mg mL−1 for sophoridine. Eleven different batches of Gan-Yan-Ling injections were analyzed and the results indicated that the method was sensitive and reliable for the determination of four alkaloids and could be used to control the quality of the preparation.


Gas chromatography-mass spectrometry Selective ion monitoring Cytisine, sophocarpine, matrine, and sophoridine Gan-Yan-Ling injection 


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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Na Chen
    • 1
  • Zheng Han
    • 1
  • Lianjun Luan
    • 1
  • Yongjiang Wu
    • 1
  1. 1.Institute of Modern Chinese Medicine, College of Pharmaceutical SciencesZhejiang UniversityHangzhouPeople’s Republic of China

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