, Volume 70, Issue 1–2, pp 109–116 | Cite as

Optimization of the Extraction Conditions and Simultaneous Quantification of Six Flavonoid Glycosides in Flos Chrysanthemi by RP-LC

  • Ya-Jun Wang
  • Xiu-Wei Yang
  • Qiao-Sheng Guo
  • Hai-Tao Liu


A sensitive, accurate and reliable reversed-phase liquid chromatographic method coupled with DAD (278 nm) was established for simultaneous quantification of six compounds in 20 cultivars of Flos Chrysanthemi. The method was carried out by using a Kromasil 100-5 C18 column with methanol–acetonitrile—1.414 × 10−2 mol L−1 aqueous phosphoric acid as a gradient mobile phase. The contents of the six flavonoid glycosides in Flos Chrysanthemi could be determined within 120 min. The linear calibration ranges for these were 0.42–126.00, 11.44–220.00, 0.53–530.00, 4.80–195.00, 11.00–220.00, and 0.12–200.00 μg mL−1. Their recoveries were 95.33–105.33% with RSDs from 0.10 to 2.00%. Their lower limits of quantification were 0.420, 1.144, 0.250, 0.480, 0.242, and 0.120 μg mL–1. The method can be used for analysis of the six flavonoid glycosides in Flos Chrysanthemi.


Column liquid chromatography Quantification Orthogonal array design Flavonoid glycosides Flos Chrysanthemi 



This project was supported by the National Sciences and Technology Program (2004BA721A20, 2006BAI06A12-11) of China.


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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  1. 1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical SciencesPeking UniversityBeijingChina
  2. 2.Institute of Chinese Medicinal MaterialsNanjing Agricultural UniversityNanjingChina
  3. 3.Institute of Medicinal Plant Development and Peking Union Medical CollegeChinese Academy of Medical Science BeijingChina

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