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Chromatographia

, Volume 69, Issue 11–12, pp 1201–1209 | Cite as

Stability-Indicating LC Method for the Determination of Lacidipine in Tablets. Application to Degradation Kinetics and Content Uniformity Testing

  • Fathalla Belal
  • Amina Elbrashy
  • Manal Eid
  • Jenny Jeehan Nasr
Original

Abstract

A simple, stability-indicating, reversed-phase liquid chromatographic method was developed for the determination of lacidipine in the presence of its degradation products. The analysis was carried out using a 150 mm × 4.6 mm i.d., 5 μm particle size Nucleodur MN-C18 column. Mobile phase containing a mixture of acetonitrile and 0.02 M phosphate buffer (70:30) at pH = 5.0 was pumped at a flow rate of 1 mL min−1 with UV-detection at 254 nm. The method showed good linearity in the range of 0.06–15 μg mL−1 with a limit of detection (S/N = 3) of 0.016 μg mL−1 (3.5 × 10−8 M). The suggested method was successfully applied for the analysis of lacidipine in bulk and in commercial tablets with average recoveries of 100.19 ± 0.81% and 100.05 ± 0.69%, respectively. The results were favorably compared to those obtained by a reference method. The suggested method was utilized to investigate the kinetics of alkaline, acidic, peroxide and photo-induced degradation of the drug. The apparent first-order rate constant, half-life times and activation energies of the degradation process were calculated. The pH profile curve was derived. The proposed method was successfully applied to the content uniformity testing of tablets.

Keywords

Column liquid chromatographic Content uniformity testing Stability-indicating and degradation-kinetics Lacidipine 

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Fathalla Belal
    • 1
  • Amina Elbrashy
    • 1
  • Manal Eid
    • 1
  • Jenny Jeehan Nasr
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of PharmacyUniversity of MansouraMansouraEgypt

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