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Chromatographia

, Volume 69, Issue 11–12, pp 1283–1289 | Cite as

An LC Fingerprint Study of Poria cocos (Schw.) Wolf

  • Xin Zhou
  • Yi-Sha Zhang
  • Yang Zhao
  • Xiao-Jian Gong
  • Chao Zhao
  • Hua-Guo Chen
Original

Abstract

The aim of the paper was to establish an efficient and accurate fingerprint method for the identification and quality control of Poria cocos (Schw.) Wolf. Methanol extracts of 43 samples of sclerotium and five samples of tegument were analysed by LC with photodiode array detection. Twenty common peaks were found and eleven of them were identified by mass spectrometry. The mutual pattern of 43 sclerotium samples was established and a Similarity Evaluation System and Principal Component Analysis were performed to analyze, differentiate and classify the samples. The method showed high precision, good repeatability and stability and all the peaks were well separated, so it can be used to assess the quality of P. cocos and to identify possible counterfeiting.

Keywords

Column liquid chromatography Mass spectrometry Chromatographic fingerprints Principal component analysis Poria cocos (Schw.) Wolf 

Notes

Acknowledgement

The authors gratefully acknowledge the financial support from the Special Support Item of Guizhou Province (No. TZJF-2006-26) and the Nomarch Foundation of Science Technology and Education of Excellent Talent of Guizhou Province (No. 2005-249).

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Xin Zhou
    • 1
    • 2
  • Yi-Sha Zhang
    • 1
    • 2
  • Yang Zhao
    • 1
    • 2
  • Xiao-Jian Gong
    • 1
    • 2
  • Chao Zhao
    • 1
    • 2
  • Hua-Guo Chen
    • 1
    • 2
  1. 1.The Research Center for Quality Control of Natural MedicineGuizhou Normal UniversityGuiyangPeople’s Republic of China
  2. 2.Key Laboratory for Information System of Mountainous Areas and Protection of Ecological EnvironmentGuiyangPeople’s Republic of China

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