Development and Validation of a Stability-Indicating LC Method for Curcumin
- 261 Downloads
A simple, isocratic, stability-indicating liquid chromatographic method for quantitative determination of curcumin was successfully developed. The chromatographic separations were achieved using a Hi-Q-Sil C18; 4.6 mm × 250 mm and 10 μm particle size column employing acetonitrile and acetate buffer (pH 3.0; 60: 40, v/v) as the mobile phase. The analyte was subjected to acidic, basic, oxidative, thermal and photo degradation. The method was validated with respect to linearity, precision, accuracy, limit of detection and limit of quantification. Curcumin was detected by UV-Vis detector at 425 nm whereas the degradation products were detected at 280 nm. The method was linear over the concentration range of 1–10 μg mL−1. The limit of detection was found to be 0.06 μg mL−1 and the quantification limit was 0.21 μg mL−1. Considerable degradation of the analyte was observed when it was subjected to alkaline conditions. Accuracy, evaluated as recovery, was in the range of 97–103%. Intra-day precision and intermediate precision showed relative standard deviations <1% and <2% respectively.
KeywordsColumn liquid chromatography Validation and forced degradation Stability-indicating Curcumin
The authors are thankful to the Konark Herbals and Healthcare Pvt. Ltd., Mumbai, India for providing the gift sample of curcumin. Prajakta Dandekar is thankful to the University Grants Commision, Delhi, India for providing the fellowship necessary for executing the work.
- 2.Araújo CAC, Leon LL (2001) Mem Inst Oswaldo Cruz Rio de Janeiro 96(5):723–728Google Scholar
- 4.Kim HY, Park EJ, Joe EH, Jou I (2003) J Immunol 171:6072–6079Google Scholar
- 6.Kiuchi F, Goto Y, Sugimoto N, Akao N, Kondo K, Tsuda Y (1993) Chem Pharm Bul 41:1640–1643Google Scholar
- 13.Šmerák A, Polívková Z, Šestáková H, Štětina R, Bárta I, Langová M, Turek B, Bártová J (2006) Czech J Food Sci 24(2):72–83Google Scholar
- 14.Shukla Y, Arora A, Taneja P (2003) Teratogen Carcin Mut 23(Suppl 1):323–335. doi: 10.1002/tcm.10059
- 23.Karasz AB, DeCocca F, Bokus L (1973) J Assoc Off Anal Chem 56:626–628Google Scholar
- 30.ICH Q2(R1) Validation of Analytical Procedures: Text and Methodology (2005) International Conference on Harmonisation of Technical requirements for Registration of Pharmaceuticals for Human Use, Geneva, SwitzerlandGoogle Scholar