Chromatographia

, Volume 69, Issue 9–10, pp 1061–1065 | Cite as

LC-MS-MS Development and Validation for Quantitative Determination of Methoxsalen in Human Plasma

  • Noel Alex Gomes
  • Vikas V. Vaidya
  • Ashutosh M. Pudage
  • Santosh S. Joshi
  • Avdhoot M. Laud
  • Snehal Basagare
  • Jayram A. Tandel
Full Short Communication

Abstract

A rapid, sensitive and specific method for quantification of methoxsalen in human plasma using metaxalone as the internal standard is presented. An Applied Biosystems Sciex API 3200 triple quadrupole mass spectrometer in multiple reaction monitoring mode, using TurboIonSpray in the positive ion mode was used. Simple solid phase extraction was followed by C18 reverse phase chromatography and tandem mass spectrometry using metaxalone as the internal standard. The mean recovery for methoxsalen was 90.0% with a lower limit of quantification of 1.0 ng mL−1. This validated assay method makes use of the sensitivity and selectivity of tandem mass spectrometry detection to allow for a more rapid and selective method for the determination of methoxsalen in human plasma.

Keywords

Column liquid chromatography Tandem mass spectrometry Human plasma Methoxsalen and metaxalone 

Notes

Acknowledgments

The authors are indebted to the directors of Accutest Research Laboratories, Navi Mumbai, India for providing the necessary facilities pertaining to this research work.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Noel Alex Gomes
    • 1
    • 2
  • Vikas V. Vaidya
    • 1
  • Ashutosh M. Pudage
    • 2
  • Santosh S. Joshi
    • 2
  • Avdhoot M. Laud
    • 2
  • Snehal Basagare
    • 2
  • Jayram A. Tandel
    • 2
  1. 1.Department of ChemistryRuia College, Mumbai UniversityMumbaiIndia
  2. 2.Accutest Research Laboratories (I) Pvt. LtdNavi MumbaiIndia

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