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Chromatographia

, Volume 69, Issue 9–10, pp 1019–1023 | Cite as

Simultaneous Determination of Etodolac and Acetaminophen in Tablet Dosage Form by RP-LC

  • Vaijanath G. Dongre
  • Sweta B. Shah
  • Gunaji S. Bayes
  • Manisha Phadke
  • Vivek K. Jadhav
Full Short Communication

Abstract

A simple, specific, precise and accurate reverse phase liquid chromatographic (RP-LC) method has been developed for the simultaneous determination of etodolac and acetaminophen in tablet dosage form. The chromatographic separation was achieved on a BDS Hypersil C18, 100 mm × 4.6 mm, 5 μm column at a detector wavelength of 274 nm using an isocratic mobile phase consisting of a mixture of 0.05% aqueous orthophosphoric acid and acetonitrile in the ratio of 50:50 (v/v) at a flow rate of 1.0 mL min−1. The retention times for etodolac and acetaminophen were found to be 1.32 and 4.24 min, respectively. The method was validated for the parameters like specificity, linearity, precision, accuracy and robustness. The method was found to be specific and stability indicating as no interfering peaks of impurities, degradent and excipients were observed. The square of correlation coefficients (R 2) for etodolac and acetaminophen were 0.9996 and 0.9998 while percentage recoveries were 101.32 and 100.94%, respectively. Intra- and inter-day relative standard deviations for both the components were <2.0%. The proposed RP-LC method can be applied for the routine analysis of commercially available formulations of these drugs either as such or in combination.

Keywords

Column liquid chromatography Validation Etodolac and acetaminophen 

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Vaijanath G. Dongre
    • 1
  • Sweta B. Shah
    • 1
    • 2
  • Gunaji S. Bayes
    • 1
  • Manisha Phadke
    • 2
  • Vivek K. Jadhav
    • 2
  1. 1.Department of ChemistryUniversity of MumbaiMumbaiIndia
  2. 2.Analytical Development LaboratoryIpca Laboratories LtdMumbaiIndia

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