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Simultaneous Determination of Etodolac and Acetaminophen in Tablet Dosage Form by RP-LC

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Abstract

A simple, specific, precise and accurate reverse phase liquid chromatographic (RP-LC) method has been developed for the simultaneous determination of etodolac and acetaminophen in tablet dosage form. The chromatographic separation was achieved on a BDS Hypersil C18, 100 mm × 4.6 mm, 5 μm column at a detector wavelength of 274 nm using an isocratic mobile phase consisting of a mixture of 0.05% aqueous orthophosphoric acid and acetonitrile in the ratio of 50:50 (v/v) at a flow rate of 1.0 mL min−1. The retention times for etodolac and acetaminophen were found to be 1.32 and 4.24 min, respectively. The method was validated for the parameters like specificity, linearity, precision, accuracy and robustness. The method was found to be specific and stability indicating as no interfering peaks of impurities, degradent and excipients were observed. The square of correlation coefficients (R 2) for etodolac and acetaminophen were 0.9996 and 0.9998 while percentage recoveries were 101.32 and 100.94%, respectively. Intra- and inter-day relative standard deviations for both the components were <2.0%. The proposed RP-LC method can be applied for the routine analysis of commercially available formulations of these drugs either as such or in combination.

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References

  1. Balfour JA, Buckley MM (1991) Drugs 42(2):274–299

    Article  CAS  Google Scholar 

  2. Merck Index (2000) 12th edn. Merck, USA, p 47

  3. Hyllested M, Jones S, Pedersen JL (2002) Br J Anaesth 88:199–214. doi:10.1093/bja/88.2.199

    Article  CAS  Google Scholar 

  4. Glowinski J (1999) Clin Drug Investig 18(3):189–197. doi:10.2165/00044011-199918030-00003

    Article  CAS  Google Scholar 

  5. McNicol E, Strassels SA, Goudas L, Lau J, Carr DB (2005) Cochrane Database Syst Rev (1):CD005180

  6. Seideman P (1993) Agents Actions Suppl 44:7–12

    CAS  Google Scholar 

  7. Silverfield JC, Kamin M, Wu SC, Rosenthal N (2002) Clin Ther 24(2):282–297. doi:10.1016/S0149-2918(02)85024-X

    Article  CAS  Google Scholar 

  8. Dionne R (2000) Compend Cont Educ Dent 21(7):572–574, 576–577

    Google Scholar 

  9. Gopinath R, Rajan S, Meyyanathan SN, Krishnaveni N, Suresh B (2007) Indian J Pharm Sci 69(1):137–140

    Article  CAS  Google Scholar 

  10. Garg G, Saraf S, Swarnalata S (2007) Indian J Pharm Sci 69(5):692–694

    Article  CAS  Google Scholar 

  11. Rao RN, Narasaraju A (2006) Anal Sci 22:287–292. doi:10.2116/analsci.22.287

    Article  CAS  Google Scholar 

  12. Momin MY, Yeole PG, Puranik MP, Wadher SJ (2006) Indian J Pharm Sci 68(3):387–389

    Article  CAS  Google Scholar 

  13. Vertzoni MV, Archontaki HA, Galanopoulou P (2003) J Pharm Biomed Anal 32(2):487–493. doi:10.1016/S0731-7085(03)00246-2

    Article  CAS  Google Scholar 

  14. Campanero MA, Calahorra B, García-Quétglas E, López-Ocáriz A, Honorato J (1999) J Pharm Biomed Anal 20:327–334. doi:10.1016/S0731-7085(99)00051-5

    Article  CAS  Google Scholar 

  15. Lau OW, Luk SF, Cheung YM (1989) Analyst (Lond) 114:1047–1051. doi:10.1039/an9891401047

    Article  CAS  Google Scholar 

  16. Chen X, Huang J, Kong Z, Zhon D (2005) J Chromatogr B Analyt Technol Biomed Life Sci 817:263–269. doi:10.1016/j.jchromb.2004.12.011

    Article  CAS  Google Scholar 

  17. Strickmann DB, Blaschke G (2001) J Pharm Biomed Anal 25:977–984. doi:10.1016/S0731-7085(01)00358-2

    Article  CAS  Google Scholar 

  18. Strickmann DB, Chankvetadze B, Blaschke G, Desiderio C, Fanali S (2000) J Chromatogr A 887:393–407. doi:10.1016/S0021-9673(99)01331-X

    Article  CAS  Google Scholar 

  19. Kousy NMEI (1999) J Pharm Biomed Anal 20:185–194. doi:10.1016/S0731-7085(99)00019-9

    Article  Google Scholar 

  20. The Official Compendia of Standards (2007) The United States Pharmacopoeia, 30th edn. USP Convention Inc., Rockville, p 2104, 1271

  21. British Pharmacopoeia (2008) vol III, International edn. HMSO Publication, London, p 2685, 2968

  22. Indian Pharmacopoeia (1996) vol II, 4th edn. Government of India, The Controller of Publications, New Delhi, p 556

  23. Dogan HN (1998) Pharmazie 53:781–784

    CAS  Google Scholar 

  24. Bhatia MS, Kaskhedikar SG, Chaturvedi SC (1997) Indian J Pharm Sci 59(2):45–48

    CAS  Google Scholar 

  25. Sharma AK, Gangwal S (1996) Indian J Pharm Sci 58(5):216–218

    Google Scholar 

  26. Ashok K, Anroop B, Vijay KS (2003) Indian Drugs 40(12):727–730

    Google Scholar 

  27. Sena MM, Freitas CB, Silva LC (2007) Quim Nova 30:75–79. doi:10.1590/S0100-40422007000100017

    CAS  Google Scholar 

  28. Xu X, Stewart JT (2000) J Liq Chromatogr Relat Technol 23:769–779. doi:10.1081/JLC-100101488

    Article  CAS  Google Scholar 

  29. Dinc F (1999) J Pharm Biomed Anal 21:723–730. doi:10.1016/S0731-7085(99)00186-7

    Article  CAS  Google Scholar 

  30. Bhushan R, Gupta D, Mukherjee A (2007) Biomed Chromatogr 21:1284–1290. doi:10.1002/bmc.885

    Article  CAS  Google Scholar 

  31. Anna W, Jin S, Haijun F, Shuo G, Zhonggui H (2008) Chromatographia 67:281–285. doi:10.1365/s10337-007-0493-2

    Article  CAS  Google Scholar 

  32. ICH Harmonized tripartite guideline (2005) Validation of analytical procedures: text and methodology Q2 (R1)

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Authors and Affiliations

  1. Department of Chemistry, University of Mumbai, Vidyanagari, Kalina Santacruz (East), Mumbai, 400098, India

    Vaijanath G. Dongre, Sweta B. Shah & Gunaji S. Bayes

  2. Analytical Development Laboratory, Ipca Laboratories Ltd, Kandivli Industrial Estate, Kandivli (West), Mumbai, 400067, India

    Sweta B. Shah, Manisha Phadke & Vivek K. Jadhav

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  1. Vaijanath G. Dongre
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  2. Sweta B. Shah
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  3. Gunaji S. Bayes
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  4. Manisha Phadke
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  5. Vivek K. Jadhav
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Correspondence to Vaijanath G. Dongre.

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Dongre, V.G., Shah, S.B., Bayes, G.S. et al. Simultaneous Determination of Etodolac and Acetaminophen in Tablet Dosage Form by RP-LC. Chroma 69, 1019–1023 (2009). https://doi.org/10.1365/s10337-009-0974-6

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  • DOI: https://doi.org/10.1365/s10337-009-0974-6

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