Advertisement

Chromatographia

, Volume 69, Issue 7–8, pp 735–742 | Cite as

Development of a Chromatographic Fingerprint of Tobacco Flavor by Use of GC and GC-MS

  • Xiaolan Zhu
  • Yun Gao
  • Zhiyan Chen
  • Qingde Su
Original

Abstract

A new chromatographic fingerprinting method has been established for quality control of tobacco flavor. Three different extraction techniques, simultaneous distillation extraction (SDE), liquid–liquid extraction, and solid-phase microextraction were evaluated for isolation of the components of interest. After comparison of their performance, a combination of SDE and GC-MS was used for simple, reliable, and reproducible development of a chromatographic fingerprint of tobacco flavor. Twelve samples of the flavor from different batches were used to establish the fingerprint. Thirty-nine volatile components of the tobacco flavor samples, accounting for 86.54% of the total content, were identified and quantified. The 12 samples had 28 peaks in common. The method of fingerprint analysis was then validated on the basis of the relative retention times and relative peak areas of the common peaks, sample stability, and similarity analysis. The similarities of the 12 samples of tobacco flavor were >0.80, showing that samples from different batches were, to some extent, consistent. The chromatographic fingerprint developed was successfully used to differentiate tobacco flavor samples from tobacco extract prepared from tobacco leaf, both by similarity comparison and by principal-components projection analysis. The method can be used for quality control of tobacco flavor.

Keywords

Gas chromatography Mass spectrometry Chromatographic fingerprint Tobacco flavor Quality control Similarity analysis 

References

  1. 1.
    Baker RR, Pereira da Silva JR, Smith G (2004) Food Chem Toxicol 42(Suppl 1):3–37. doi: 10.1016/S0278-6915(03)00189-3 Google Scholar
  2. 2.
    Gaworski CL, Heck JD, Bennett MB, Wenk ML (1999) Toxicology 139:1–17. doi: 10.1016/S0300-483X(99)00094-3 CrossRefGoogle Scholar
  3. 3.
    Yu JJ (2003) Cigarette technology. Chinese Agriculture Press, Beijing, p 95Google Scholar
  4. 4.
    Guo FQ, You J, Zhong KJ, Huang JG, Liang YZ, Huang KL (2005) J Cent South Univ Sci Tech (Paris) 36:828–836Google Scholar
  5. 5.
    Zhu M, Cao Y, Fan GR (2007) J Sep Sci 30:67–73. doi: 10.1002/jssc.200600261 CrossRefGoogle Scholar
  6. 6.
    Hou Y, Yang L, Wang BX, Xu JC, Yang Y, Yang Y, Cao Q, Xie X (2006) Chin J Chromatogr A 24:601–605CrossRefGoogle Scholar
  7. 7.
    Ferhat MA, Tigrine-Kordjani N, Chemat S, Meklati BY, Chemat F (2007) Chromatographia 65:217–222. doi: 10.1365/s10337-006-0130-5 CrossRefGoogle Scholar
  8. 8.
    Careri M, Manini P, Spaynoli S, Barbieri G, Bolzoni L (1994) Chromatographia 38:386–394. doi: 10.1007/BF02269785 CrossRefGoogle Scholar
  9. 9.
    Gan HL, Che Man YB, Tan CP, NorAini I, Nazimah SAH (2005) Food Chem 89:507–518. doi: 10.1016/j.foodchem.2004.03.005 CrossRefGoogle Scholar
  10. 10.
    Stanfill SB, Ashley DL (1999) J Chromatogr A 858:79–89. doi: 10.1016/S0021-9673(99)00796-7 CrossRefGoogle Scholar
  11. 11.
    Cai JB, Liu BZ, Ling Q, Su QD (2002) J Chromatogr A 947:267–275. doi: 10.1016/S0021-9673(02)00015-8 CrossRefGoogle Scholar
  12. 12.
    Hu K, Zhao SL, Ye FG, Lu X (2008) Chromatographia. doi: 10.1365/s10337-008-0702-7
  13. 13.
    Gu M, Yang F, Su ZG (2004) J Chromatogr A 1022:139–144. doi: 10.1016/j.chroma.2003.09.038 CrossRefGoogle Scholar
  14. 14.
    Niu F, Niu ZQ, Xie GB, Meng F, Zhang GE, Cui Z, Tu PF (2006) Chromatographia 64:593–597. doi: 10.1365/s10337-006-0069-6 CrossRefGoogle Scholar
  15. 15.
    Tong L, Wang YZ, Xiong JF, Cui Y, Zhou YG, Yi LC (2008) Talanta 76:80–84. doi: 10.1016/j.talanta.2008.02.012 CrossRefGoogle Scholar
  16. 16.
    Wang LC, Cao YH, Xing XP, Ye JN (2005) Chromatographia 62:283–288. doi: 10.1365/s10337-005-0624-6 CrossRefGoogle Scholar
  17. 17.
    Schultz H, Flath RA (1977) J Agric Food Chem 25:446–450. doi: 10.1021/jf60211a038 CrossRefGoogle Scholar
  18. 18.
    Cai JB, Liu BZ, Su QD (2001) J Chromatogr A 930:1–7. doi: 10.1016/S0021-9673(01)01187-6 CrossRefGoogle Scholar
  19. 19.
    Chen Y, Yan Y, Xie MY, Nie SP, Liu W, Gong XF, Wang YX (2008) J Pharm Biomed Anal 47:469–477. doi: 10.1016/j.jpba.2008.01.039 CrossRefGoogle Scholar
  20. 20.
    Huang LF, Wu MJ, Zhong KJ, Sun ZJ, Liang YZ, Dai YH, Huang KL, Guo FQ (2007) Anal Chim Acta 588:216–223. doi: 10.1016/j.aca.2007.02.013 CrossRefGoogle Scholar
  21. 21.
    Liu AH, Lin YH, Yang M, Guo H, Guan SH, Sun JH, Guo DA (2007) J Chromatogr B Anal Technol Biomed Life Sci 846:32–41. doi: 10.1016/j.jchromb.2006.08.002 CrossRefGoogle Scholar
  22. 22.
    Cao YH, Wang LC, Xu YJ, Ye JN (2006) J Pharm Biomed Anal 41:845–856. doi: 10.1016/j.jpba.2006.01.060 CrossRefGoogle Scholar
  23. 23.
    Xie PS, Chen SB, Liang YZ, Wang XH, Tian RT, Upton R (2006) J Chromatogr A 1112:171–180. doi: 10.1016/j.chroma.2005.12.091 CrossRefGoogle Scholar
  24. 24.
    Zhang YY, Dan M, Wu JB, Yang HZ, Huang H, Ying Q, Wei SD, Okuyama T, Nakajima K (2008) Chromatographia 68:101–104. doi: 10.1365/s10337-008-0653-z CrossRefGoogle Scholar
  25. 25.
    Yang J, Chen LH, Zhang Q, Lai MX, Wang Q (2007) J Sep Sci 30:1276–1283. doi: 10.1002/jssc.200600389 CrossRefGoogle Scholar
  26. 26.
    Kanya Z, Forgacs E, Cserhati T, Illes Z (2006) Chromatographia 63:129–134. doi: 10.1365/s10337-005-0687-4 CrossRefGoogle Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  1. 1.Research Center of Tobacco and HealthUniversity of Science and Technology of ChinaHefeiChina
  2. 2.Technical CenterChina Tobacco Guangxi Industrial CorporationNanningChina
  3. 3.Department of ChemistryUniversity of Science and Technology of ChinaHefeiChina

Personalised recommendations