Abstract
Pomiferin and osajin are major bioactive isoflavones found in Maclura pomifera (Rafin.) Schneider (Moraceae). A simple, reproducible, and sensitive method for analysis of pomiferin and osajin in different parts of M. pomifera has been developed and validated. The compounds were separated on a C8 column by gradient elution at a flow rate at 1.0 mL min−1. Column eluent was monitored at 280 nm by use of a diode-array detector. Linear range, peak purity, selectivity, system performance, precision, accuracy, and robustness were shown to be acceptable. LC–MS with atmospheric pressure electrospray ionization was used for identification of the compounds; use of positive-ion mode resulted in protonated pseudomolecular ions of the compounds.
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Acknowledgment
M. Abu-Asaker would like to acknowledge a doctoral grant from the Turkish Scientific and Technical Research Council (TUBITAK).
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Kartal, M., Abu-Asaker, M., Dvorska, M. et al. LC–DAD–MS Method for Analysis of Pomiferin and Osajin, Major Isoflavones in Maclura pomifera (Rafin.) Schneider. Chroma 69, 325–329 (2009). https://doi.org/10.1365/s10337-008-0900-3
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DOI: https://doi.org/10.1365/s10337-008-0900-3