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Chromatographia

, Volume 69, Issue 3–4, pp 339–344 | Cite as

Analysis of (−)-Shikimic Acid in Chinese Star Anise by GC–MS with Selected Ion Monitoring

  • Hongcheng Liu
  • Qiwan Li
  • Yin Zhang
  • Yanhong Zhou
Full Short Communication

Abstract

A sensitive, specific, accurate, and reproducible GC–MS method with selected ion monitoring for quantitative determination of (−)-shikimic acid (SA) in Chinese star anise, using benzoic acid as internal standard, has been developed and validated. The homogeneity of the sample was evaluated by one way analysis of variance. The extraction efficiency of ultrasound-assisted and Soxhlet extraction was compared. Results showed that ultrasonic extraction was as efficient as Soxhlet extraction but was more rapid and simpler. Linearity and recovery were good. The method was used for analysis of (−)-SA in Chinese star anise from different areas in Wenshan state, Yunnan province. It was found that Chinese star anise collected from Pingbian and Funing counties contained more (−)-SA than other samples, with two plants from Pingbian county containing >15% (−)-SA.

Keywords

Gas chromatography-mass spectrometry Selected ion monitoring (−)-Shikimic acid Chinese star anise (Illiciumverum Hook. f.) 

Notes

Acknowledgments

We thank Mo Shunyan and Dong LinLin, now working on natural products at the University of Illinois at Chicago, for their discussion and revision of this paper.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Hongcheng Liu
    • 1
    • 2
  • Qiwan Li
    • 1
  • Yin Zhang
    • 2
  • Yanhong Zhou
    • 1
  1. 1.Supervision and Testing Center for Farm Product Quality, Ministry of AgricultureQuality Standard and Testing Technology Institute, Yunnan Academy of Agricultural ScienceKunmingPeople’s Republic of China
  2. 2.College of Biosystem Engineering and Food ScienceUniversity of ZhejiangHangzhouPeople’s Republic of China

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