Abstract
A liquid chromatography method was developed and validated for the determination of picroside-I and picroside-II in herbal preparations containing Picrorhiza kurroa as one of the ingradients. Resolution of picrosides was achieved on a reversed phase (C-18) endcapped bidentate column by using a mobile phase of acetonitrile: water (25:75 v/v). The detection of picrosides was carried out at 270 nm. The method was validated for precision, accuracy and robustness according to the International Conference on Harmonization (ICH) guidelines and is applicable for the quality control of preparations containing P. kurroa. Analysis of samples in forced degradation proved it to be applicable for stability evaluation. The linear regression analysis data showed good linear relationship (r 2 = 0.9999 ± 0.0010 for picroside-I and 0.9997 ± 0.0012 for picroside-II) in the concentration range of 0.4–4.0 μg. The limit of detection and quantification for picroside-I and picroside-II were recorded to be 28.1 and 73.1 ng and 85.2 and 221.5 ng, respectively. Satisfactory recovery results were observed from the herbal preparations (97.5–100.5%). Intra- and inter- day precision of the method was acceptable, with relative standard deviation (%RSD) values in the range of 0.04–1.16% and 0.03–0.27%, respectively.
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Nadkarni AK (1954) Indian Materica, vol vol. 1. Popular Book Dept, Bombay, p 25–27, 619–622, 634–651, 953–955, 1220–1221, 1252–1253
Ansari RA, Oswal BS, Chandra R, Dhawan BN, Garg NK, Kapoor NK, Kulshrestha DK, Mendhi H, Mehrotra BN, Patnaik GK, Sharma SK (1988) J Med Res 47:401–404
Chaturvedi GN, Singh RH (1996) J Res Indian Med 1:1–14
Langer JG, Gupta OP, Atal CK (1981) Indian J Pharmacol 13:98–99
Kirtikar KR, Basu BD (1935) Indian medicinal plants, vol III. Lalit Mohan Basu, Allahabad, pp 1825–1826
Dey AC, Bhishen S, Mahendra PS (1980) Indian medicinal plants used in ayurvedic preparations. Dehradun, India. J Res Indian Med p 81
Kumar SHS, Anandan R, Devki T, Kumar MS (2001) Fitoterapia 72:402–405. doi:10.1016/S0367-326X(01)00264-7
Atal CK, Sharma ML, Kaul A, Khjuria A (1986) J Ethnopharmacol 18:133–141. doi:10.1016/0378-8741(86)90025-5
Puri A, Saxena RP, Sumti Guru PY, Kulshrestha DK, Dhawan BN (1992) Planta Medica 58:519–523. doi:10.1055/s-2006-961542
Sharma ML, Rao CS, Duda PL (1994) J Ethnopharmacol 41:185–192. doi:10.1016/0378-8741(94)90031-0
Kitagawa I, Hino K, Nishimura T, Iwata E, Yosioka I (1971) Chem Pharm Bull (Tokyo) 19:2534–2544
Weinges K, Kloss P, Henkels WD (1972) Anal Chem 759:173–182
Halliwel B, Gutteridge MC (1999) Free Radicals in biology and medicine. In study of generalized light emission, 3rd edn. University Press, Oxford, p 387
Singh GB, Bani S, Singh S, Khajuria A, Sharma ML, Gupta BD, Banerjee SK (1993) Phytother Res 7:402–407. doi:10.1002/ptr.2650070604
Joy KL, Kuttan R (1999) J Ethnopharmacol 167:143–148. doi:10.1016/S0378-8741(98)00243-8
Vaidya AB, Antakar DS, Doshi JC, Bhatt AD, Ramesh V, Vora PV, Perissond D, Baxi AJ, Kale PM (1996) J Postgrad Med 42:105–108
Sturm S, Stuppner H (2000) Phytochem Anal 11:121–127 10.1002/(SICI)1099-1565(200003/04)11:2<121::AID-PCA493>3.0.CO;2-9
Stuppner H, Wagner H (1992) Sci Pharm 60:73–85
Kumar V, Mehrotra N, Lal J, Gupta RC (2004) J Chromatogr A 1045:145–152. doi:10.1016/j.chroma.2004.06.021
Singh N, Gupta AP, Singh B, Kaul VK (2005) J Liq Chromatgr Relat Tecchnol 28:1679–1691. doi:10.1081/JLC-200060439
Bansal M, Sharma PK, Jain GK, Mehrotra N (2007) Intern. J Chem Sci 5(2):852–866
Shan C, Haung J, Junqi H (2007) Pharmazie 62(8):577–579
Sturm S, Stuppner H (2001) Chromatographia 53:612–618. doi:10.1007/BF02493007
Chauhan SK, Singh BP, Agrawal SR (1999) Indian Drugs 36:604–605
Dwivedi AK, Chaudhry M, Seth RK, Sarin J (1968) Indian J Pharm Sci 51:274–275
Bhandari P, Kumar N, Singh B, Kaul VK (2008) J Chrom A 1194:254–261. doi:10.1016/j.chroma.2008.04.062
Kumar V, Mehrotra N, Gupta RC (2005) J Chrom B 820:221–227. doi:10.1016/j.jchromb.2005.03.032
Singh S, Singh B, Bahuguna R, Wadhwa L, Saxena R (2006) J Pharm Biomed Anal 41:1037–1040. doi:10.1016/j.jpba.2006.01.030
Mohammadi A, Haririan I, Rezanour N, Ghiasi L, Walker RB (2006) J Chromatogr A 1116:153–157. doi:10.1016/j.chroma.2006.03.038
Ivana I, Ljiljana Z, Mira Z (2006) J Chromatogr A 1119:209–215. doi:10.1016/j.chroma.2005.12.079
Annon., ICH, Q1A(R2) Stability testing of new drug substances and products. In: International Conference on Harmonization, IFPMA, Geneva, 2003
Grosa G, Grosso ED, Russo R, Allegrone G (2006) J Pharm Biomed Anal 41:798–803. doi:10.1016/j.jpba.2006.01.026
Mishal A, Sober D (2005) J Pharm Biomed Anal 39:819–823. doi:10.1016/j.jpba.2005.05.029
Singh B, Rastogi RP (1972) Indian J Chem 10:29–31
Jia Q, Hong MF, Minter D (1999) J Nat Prod 62:901–903. doi:10.1021/np980493+
Stuppner H, Wagner H (1989) Planta Medica 55:467–469. doi:10.1055/s-2006-962066
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Authors are grateful to the CSIR-EMR for awarding the senior research fellowship to Ms. P.B. and technical assistance from Mr. Ramesh Kumar is gratefully acknowledged.
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Bhandari, P., Kumar, N., Singh, B. et al. Stability-Indicating LC–PDA Method for Determination of Picrosides in Hepatoprotective Indian Herbal Preparations of Picrorhiza kurroa . Chroma 69, 221–227 (2009). https://doi.org/10.1365/s10337-008-0889-7
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DOI: https://doi.org/10.1365/s10337-008-0889-7