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Separation of Enantiomers and Control of Elution Order of β-Lactams by GC Using Cyclodextrin-Based Chiral Stationary Phases

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Abstract

Enantiomers of 19 racemic β-lactams, with 3 and 4-position substitutions, were separated using gas chromatography. Excellent results were achieved on derivatized cyclodextrin-based GC chiral stationary phases (CSPs). All 19 compounds were baseline separated, most with high resolution factors. The Chiraldex G-TA was found to be the most powerful CSP with the broadest enantioselectivity, while Chiraldex B-DM produced the fastest separations for most of the compounds assayed. Results obtained in this work suggest that GC can serve as a potential method for the enantiomeric separation of sufficiently volatile solid β-lactams.

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Acknowledgments

We gratefully acknowledge the support from National Institute of Health (GM053825-11) and OTKA grant K 67573 for this work.

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Authors and Affiliations

  1. Chemistry and Biochemistry Department, University of Texas at Arlington, Arlington, TX, 76019, USA

    Ke Huang & Daniel W. Armstrong

  2. Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary

    Eniko Forro & Ferenc Fulop

  3. Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary

    Antal Peter

Authors
  1. Ke Huang
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  2. Daniel W. Armstrong
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  3. Eniko Forro
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  4. Ferenc Fulop
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  5. Antal Peter
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Corresponding author

Correspondence to Daniel W. Armstrong.

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Huang, K., Armstrong, D.W., Forro, E. et al. Separation of Enantiomers and Control of Elution Order of β-Lactams by GC Using Cyclodextrin-Based Chiral Stationary Phases. Chroma 69, 331–337 (2009). https://doi.org/10.1365/s10337-008-0888-8

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