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Development of a Sensitive LC Assay with Fluorescence Detection for the Determination of Zearalenone in Rat Serum

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Abstract

A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid–liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL−1 (r = 0.9995), with the limit of quantification at 10 ng mL−1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.

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Acknowledgements

This work was supported by Korea Food & Drug Administration Grant No. 08182–488.

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Authors and Affiliations

  1. College of Pharmacy, Catholic University of Daegu, Gyeongsan-si, Gyeongbuk, South Korea

    Beom Soo Shin

  2. College of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do, South Korea

    Seok Hyun Hong, Hyoung Jun Kim, Sang Wook Hwang, Jong Bong Lee & Sun Dong Yoo

  3. Human Exposure Assessment Division, National Institute of Toxicological Research, Eunpyung-ku, Seoul, South Korea

    Hae-Seong Yoon & Do Jung Kim

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  1. Beom Soo Shin
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  2. Seok Hyun Hong
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  7. Jong Bong Lee
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  8. Sun Dong Yoo
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Correspondence to Sun Dong Yoo.

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Shin, B.S., Hong, S.H., Kim, H.J. et al. Development of a Sensitive LC Assay with Fluorescence Detection for the Determination of Zearalenone in Rat Serum. Chroma 69, 295–299 (2009). https://doi.org/10.1365/s10337-008-0871-4

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  • DOI: https://doi.org/10.1365/s10337-008-0871-4

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