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LC Method for Quantification of Lutein in Rat Plasma: Validation, and Application to a Pharmacokinetic Study

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Abstract

A reversed-phase LC method has been developed for quantitative analysis of lutein in rat plasma and applied to a study of the pharmacokinetics of lutein in rats. From a variety of compounds and solvents tested, astaxanthin was selected as the internal standard. n-Hexane was found to be the best solvent for extracting lutein from plasma. LC analysis of the extracts was performed on a C18 column equipped with a guard pre-column. Linearity was good (r > 0.99) over the range 10–100 ng mL−1. Recovery from plasma was 82.7–92.9% the intra-day and inter-day precision were always better than 3%. The limits of detection (LOD) and quantification (LOQ) were 2.5 and 8.3 ng mL−1, respectively. The LC method was used to quantify lutein and zeaxanthin in rat plasma in a 36-h pharmacokinetic study in which experimental rats received a single oral dose of lutein (20 mg kg−1). The results are presented.

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Acknowledgments

The authors are grateful to Ekomir Company (Moscow, Russia) for financial support.

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Authors and Affiliations

  1. Saint Petersburg Institute of Pharmacy, 47/33, Piskarevskiy pr., 195067, St. Petersburg, Russia

    Marina V. Karlina, Olga N. Pozharitskaya, Alexander N. Shikov, Marina N. Makarova & Valery G. Makarov

  2. Interregional Center “Adaptogen”, 47/5, Piskarevskiy pr., 195067, St. Petersburg, Russia

    Olga N. Pozharitskaya, Alexander N. Shikov, Vera M. Kosman & Valery G. Makarov

Authors
  1. Marina V. Karlina
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  2. Olga N. Pozharitskaya
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  3. Alexander N. Shikov
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  4. Vera M. Kosman
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  5. Marina N. Makarova
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  6. Valery G. Makarov
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Correspondence to Alexander N. Shikov.

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Karlina, M.V., Pozharitskaya, O.N., Shikov, A.N. et al. LC Method for Quantification of Lutein in Rat Plasma: Validation, and Application to a Pharmacokinetic Study. Chroma 68, 949–954 (2008). https://doi.org/10.1365/s10337-008-0804-2

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  • DOI: https://doi.org/10.1365/s10337-008-0804-2

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