LC Method for Quantification of Lutein in Rat Plasma: Validation, and Application to a Pharmacokinetic Study
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A reversed-phase LC method has been developed for quantitative analysis of lutein in rat plasma and applied to a study of the pharmacokinetics of lutein in rats. From a variety of compounds and solvents tested, astaxanthin was selected as the internal standard. n-Hexane was found to be the best solvent for extracting lutein from plasma. LC analysis of the extracts was performed on a C18 column equipped with a guard pre-column. Linearity was good (r > 0.99) over the range 10–100 ng mL−1. Recovery from plasma was 82.7–92.9% the intra-day and inter-day precision were always better than 3%. The limits of detection (LOD) and quantification (LOQ) were 2.5 and 8.3 ng mL−1, respectively. The LC method was used to quantify lutein and zeaxanthin in rat plasma in a 36-h pharmacokinetic study in which experimental rats received a single oral dose of lutein (20 mg kg−1). The results are presented.
KeywordsColumn liquid chromatography Lutein Pharmacokinetics Rat plasma
The authors are grateful to Ekomir Company (Moscow, Russia) for financial support.
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