Development and Validation of an Accurate LC Method for the Quantitative Determination of Voriconazole in a New Emulsion Formulation
- 61 Downloads
A simple, sensitive and accurate liquid chromatographic method with UV detection was developed and validated to determine voriconazole in a new emulsion formulation. Chromatographic separation was achieved on a Diamonsil C18 column (250 × 4.6 mm I.D., 5 μm) using a mobile phase consisting of acetonitrile-water-acetic acid (40:60:0.25, v/v/v) at a flow rate of 1.0 mL min−1. The UV detection wavelength was set at 256 nm. The linear calibration curves were obtained in the concentration range of 1.00–100 μg mL−1 with the limit of quantification of 1.00 μg mL−1. The within- and between-run precisions in terms of percentage relative standard deviation were lower than 7.4 and 7.1%, respectively. The accuracy in terms of percentage relative error ranged from −1.5 to 1.4%. This validated method was successfully applied to the determination of the content of voriconazole in a new emulsion formulation.
KeywordsColumn liquid chromatography Voriconazole Emulsion
- 2.Barry ALSD (1996) Antimicrob Agents Chemother 40:1948–1949Google Scholar
- 3.Murphy M, Bernard EM, Ishimaru T, Armstrong D (1997) Antimicrob Agents Chemother 41:696–698Google Scholar
- 4.Sanati H, Belanger P, Fratti R, Ghannoum M (1997) Antimicrob Agents Chemother 41:2492–2496Google Scholar
- 5.Martin MV, Yates J, Hitchcock CA (1997) Antimicrob Agents Chemother 41:13–16Google Scholar