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Chromatographia

, Volume 68, Issue 3–4, pp 259–262 | Cite as

LC-Fluorescence Detection of Abamectin, Ivermectin, Doramectin, and Eprinomectin in Rabbit Feces

  • Haiyang Jiang
  • Degang Zhou
  • Haiyan Li
  • Fei Xu
  • Cun Li
  • Jianzhong Shen
  • Xiaowei Li
  • Shuanyang Ding
Full Short Communication

Abstract

A simple multi-residue method was developed for the simultaneous determination of abamectin, ivermectin, doramectin, and eprinomectin in rabbit feces. Samples were extracted with acetonitrile, cleaned up with a C18 solid-phase extraction cartridge, and analysed by LC with fluorescence detection after derivatization. Abamectin, ivermectin, doramectin, and eprinomectin were detected at levels of 2–500 ng g−1; the average recoveries ranged from 73.2 to 99.6% with relative standard deviations of 2.5–11.3%. The limits of detection were 0.1–0.4 ng g−1.

Keywords

Column liquid chromatography Fluorescence detection Derivatization Avermectins Rabbit feces 

Notes

Acknowledgments

The project was founded by Mega-Projects of Science Research for the 10th Five-Year Plan (No. 2006BAD31B09). This work is supported by Development Program of China during the 11th Five-Year Plan Period (No. 2006BAK10B09 and 2006BAK02A09). The authors appreciate the cooperation of other faculty members in the Department of Pharmacology and Toxicology of the Veterinary Medicine at China Agriculture University.

References

  1. 1.
    Shoop WL, Mrozik H, Fisher MH (1995) Vet Parasitol 59:139–156. doi: 10.1016/0304-4017(94)00743-V CrossRefGoogle Scholar
  2. 2.
    Schenck FJ, Lagman LH (1999) J AOAC Int 82:1340–1344Google Scholar
  3. 3.
    Holste JE, Smith LL, Hair JA, Lancaster JL, Lloyd JE, Langholff WK, Barrick RA, Eagleson JS (1997) Vet Parasitol 73:153–161. doi: 10.1016/S0304-4017(97)00063-0 CrossRefGoogle Scholar
  4. 4.
    Williams JC, Loyacano AF, DeRosa A, Gurie J, Clymer BC, Guerino FA (1999) Vet Parasitol 85:277–288. doi: 10.1016/S0304-4017(99)00121-1 CrossRefGoogle Scholar
  5. 5.
    Halley BA, VandenHeuvel WJA, Wislocki PG (1993) Vet Parasitol 48:109. doi: 10.1016/0304-4017(93)90149-H CrossRefGoogle Scholar
  6. 6.
    Herd R (1995) Int J Parasitol 25:875. doi: 10.1016/0020-7519(95)00018-W CrossRefGoogle Scholar
  7. 7.
    Spratt DM (1997) Int J Parasitol 27:173. doi: 10.1016/S0020-7519(96)00147-6 CrossRefGoogle Scholar
  8. 8.
    Suarez VH (2002) Vet Res 33:563. doi: 10.1051/vetres:2002039 CrossRefGoogle Scholar
  9. 9.
    Floate KD (2006) Can J Vet Res 70:1–10Google Scholar
  10. 10.
    Halley BA, Winter R, Yoon S, Marley SE, Rehbein S (2005) Vet Parasitol 128:109–114. doi: 10.1016/j.vetpar.2004.11.007 CrossRefGoogle Scholar
  11. 11.
    Salisbury CDC (1993) J AOAC Int 76:1149–1151Google Scholar
  12. 12.
    Payne ID, Hicks MB, Wehner TA (1995) J Agric Food Chem 43:1233–1237. doi: 10.1021/jf00053a021 CrossRefGoogle Scholar
  13. 13.
    Asbakk E, Bendiksen HR, Oksanen A (1999) J Agric Food Chem 47:999–1003. doi: 10.1021/jf980919q CrossRefGoogle Scholar
  14. 14.
    Perez R, Cabezas I, Sutra JF (2001) Vet J 161:85–92. doi: 10.1053/tvjl.2000.0521 CrossRefGoogle Scholar
  15. 15.
    Kolar L, Kuzner J, Erzen NK (2004) Biomed Chromatogr 18:117–124. doi: 10.1002/bmc.306 CrossRefGoogle Scholar
  16. 16.
    Jiang HY, Ding SY, Xu F, Zhao SJ, He JH, Liu JF, Hou XL, Shen JZ (2007) Chromatographia 66:411–414. doi: 10.1365/s10337-007-0326-3 CrossRefGoogle Scholar
  17. 17.
    Curtis SK, Housley R, Brooks DL (1990) J Am Vet Med Assoc 196:1139–1140Google Scholar
  18. 18.
    Wright FC, Riner JC (1985) Am J Vet Res 46:752–754Google Scholar
  19. 19.
    Pan BL, Wang M, Xu FR, Wang YW, Dong YT, Pan ZD (2006) Vet Parasitol 137:386–390. doi: 10.1016/j.vetpar.2006.01.013 CrossRefGoogle Scholar
  20. 20.
    Huseyin V, Bulent U, Hasan E, Tulin K, Goksel B (2005) Vet Dermatol 16:285–288. doi: 10.1111/j.1365-3164.2005.00446.x CrossRefGoogle Scholar
  21. 21.
    Pandey VS (1989) British Vet J 145:54–56Google Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Haiyang Jiang
    • 1
  • Degang Zhou
    • 1
  • Haiyan Li
    • 1
  • Fei Xu
    • 1
  • Cun Li
    • 1
  • Jianzhong Shen
    • 1
  • Xiaowei Li
    • 1
  • Shuanyang Ding
    • 1
  1. 1.Department of Pharmacology and ToxicologyCollege of Veterinary Medicine, China Agricultural UniversityBeijingPeople’s Republic of China

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