Chromatographia

, Volume 68, Issue 1–2, pp 27–31 | Cite as

Synthesis, Characteristics and Evaluation of a New Monolithic Silica Column Prepared from Copolymer Pluronic F127

  • Gengliang Yang
  • Fengxin Li
  • Lijuan Wang
  • Kyung Ho Row
  • Haiyan Liu
  • Ligai Bai
  • Weimin Cao
  • Tao Zhu
Original

Abstract

Monolithic silica columns were prepared via a sol–gel process using tri-block copolymer Pluronic F127 as structure-directing reagent. In this reaction, F127 induced phase separation but also acted as a template leading to a continuous structure of silica skeletons and textural pores as well as the formation of mesopores. The morphology of the monolithic materials was studied by scanning electron microscopy. The capability of separation and loading on this column were demonstrated. Phosphatidylcholine was separated from soy lecithin, demonstrating fast and efficient performance when the column was used for normal phase liquid chromatography.

Keywords

Column liquid chromatography Normal phase separations Monolithic silica columns Copolymer Pluronic F127 

Notes

Acknowledgments

The authors gratefully appreciate the financial support by the National Natural Science Foundation of China (20375010, 20675084), the funds from Hebei Province Programs for Science and Technology Development (NO. 06276479B, 07276407D) and “Bai Ren Project” of Chinese Academy of Sciences.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Gengliang Yang
    • 1
    • 2
  • Fengxin Li
    • 1
  • Lijuan Wang
    • 1
  • Kyung Ho Row
    • 3
  • Haiyan Liu
    • 1
  • Ligai Bai
    • 1
  • Weimin Cao
    • 1
  • Tao Zhu
    • 1
  1. 1.College of PharmacyHebei UniversityBaodingChina
  2. 2.Institutes of ChemistryChinese Academy of SciencesBeijingChina
  3. 3.Center for Advanced Bioseparation Technology, Department of Chemical EngineeringInha UniversityIncheonSouth Korea

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