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LC-MS Determination and Pharmacokinetic Study of Luteolin-7-O-β-d-glucoside in Rat Plasma after Administration of the Traditional Chinese Medicinal Preparation Kudiezi Injection

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Abstract

A rapid and sensitive LC-MS method has been developed for the determination of luteolin-7-O-β-d-glucoside in rat plasma after solvent extraction. Separation was on an Elite Hypersil ODS2 column (250 mm × 4.6 mm i.d., 5 μm) with a mobile phase of acetonitrile-0.3% acetic acid (26:74, v/v). The samples were analyzed by using positive electrospray ionization MS in selected ion monitoring mode. The selected ions for luteolin-7-O-β-d-glucoside and the internal standard, isoquercitrin, were m/z 448.95 and m/z 464.95. Good linearity was observed over the range of 20–2,000 ng mL−1 with a lower limit of quantification of 20 ng mL−1. No interference peaks or matrix effects were observed. The validated method was applied to the pharmacokinetic study of luteolin-7-O-β-d-glucoside in rat plasma after intravenous administration of Kudiezi Injection.

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning, 110016, People’s Republic of China

    Ran Yin, Xiaohui Chen, Fei Han, Zhenduo Shen, Weiming Cheng & Kaishun Bi

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  1. Ran Yin
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  2. Xiaohui Chen
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  3. Fei Han
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  4. Zhenduo Shen
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  5. Weiming Cheng
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  6. Kaishun Bi
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Correspondence to Kaishun Bi.

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Yin, R., Chen, X., Han, F. et al. LC-MS Determination and Pharmacokinetic Study of Luteolin-7-O-β-d-glucoside in Rat Plasma after Administration of the Traditional Chinese Medicinal Preparation Kudiezi Injection. Chroma 67, 961–965 (2008). https://doi.org/10.1365/s10337-008-0637-z

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  • DOI: https://doi.org/10.1365/s10337-008-0637-z

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