Chromatographia

, Volume 67, Issue 11–12, pp 961–965 | Cite as

LC-MS Determination and Pharmacokinetic Study of Luteolin-7-O-β-d-glucoside in Rat Plasma after Administration of the Traditional Chinese Medicinal Preparation Kudiezi Injection

  • Ran Yin
  • Xiaohui Chen
  • Fei Han
  • Zhenduo Shen
  • Weiming Cheng
  • Kaishun Bi
Full Short Communication

Abstract

A rapid and sensitive LC-MS method has been developed for the determination of luteolin-7-O-β-d-glucoside in rat plasma after solvent extraction. Separation was on an Elite Hypersil ODS2 column (250 mm × 4.6 mm i.d., 5 μm) with a mobile phase of acetonitrile-0.3% acetic acid (26:74, v/v). The samples were analyzed by using positive electrospray ionization MS in selected ion monitoring mode. The selected ions for luteolin-7-O-β-d-glucoside and the internal standard, isoquercitrin, were m/z 448.95 and m/z 464.95. Good linearity was observed over the range of 20–2,000 ng mL−1 with a lower limit of quantification of 20 ng mL−1. No interference peaks or matrix effects were observed. The validated method was applied to the pharmacokinetic study of luteolin-7-O-β-d-glucoside in rat plasma after intravenous administration of Kudiezi Injection.

Keywords

Column liquid chromatography-mass spectrometry Pharmacokinetic studies Luteolin-7-O-β-d-glucoside Kudiezi Injection Ixeris sonchifolia 

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Ran Yin
    • 1
  • Xiaohui Chen
    • 1
  • Fei Han
    • 1
  • Zhenduo Shen
    • 1
  • Weiming Cheng
    • 1
  • Kaishun Bi
    • 1
  1. 1.School of PharmacyShenyang Pharmaceutical UniversityShenyangPeople’s Republic of China

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