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LC–UV Method with Pre-Column Derivatization for the Determination of Ciclopirox Olamine in Raw Material and Topical Solution

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Abstract

A rapid, simple and sensitive liquid chromatographic method has been developed to assay ciclopirox olamine in raw material and topical solution. The analysis used a reversed-phase C18 end-capped column, with UV detection at 305 nm and pre-column derivatization with dimethyl sulphate. A linear response (r 2 > 0.999) was observed in the range of 0.4–200 μg mL−1. The method showed good recoveries for the raw material and topical solution and the relative standard deviation intra and inter-day were ≤2.00%. Validation parameters such as specificity and robustness were also determined. The proposed method provided an accurate and precise analysis of ciclopirox olamine in raw material and topical solution.

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Acknowledgments

The authors would like to thank the Comissão Permanente de Revisão da Farmacopéia Brasileira for the financial support.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, UFSM, Caixa Postal 5083, CEP 97110-970, Santa Maria-RS, Brazil

    Ana Laura V. Escarrone & Celso F. Bittencourt

  2. Centro Universitário Franciscano, UNIFRA, Rua dos Andradas, 1614, Santa Maria-RS, Brazil

    Luciane V. Laporta & Marcos R. dos Santos

  3. Fundação Universidade de Rio Grande, FURG, Avenida Itália, Km 8, Campus Carreiros, CEP 96201-900, Rio Grande-RS, Brazil

    Ednei G. Primel & Sergiane S. Caldas

Authors
  1. Ana Laura V. Escarrone
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  2. Celso F. Bittencourt
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  3. Luciane V. Laporta
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  4. Marcos R. dos Santos
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  5. Ednei G. Primel
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  6. Sergiane S. Caldas
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Corresponding author

Correspondence to Ana Laura V. Escarrone.

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Escarrone, A.L.V., Bittencourt, C.F., Laporta, L.V. et al. LC–UV Method with Pre-Column Derivatization for the Determination of Ciclopirox Olamine in Raw Material and Topical Solution. Chroma 67, 967–971 (2008). https://doi.org/10.1365/s10337-008-0623-5

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  • DOI: https://doi.org/10.1365/s10337-008-0623-5

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