, Volume 67, Issue 11–12, pp 967–971 | Cite as

LC–UV Method with Pre-Column Derivatization for the Determination of Ciclopirox Olamine in Raw Material and Topical Solution

  • Ana Laura V. Escarrone
  • Celso F. Bittencourt
  • Luciane V. Laporta
  • Marcos R. dos Santos
  • Ednei G. Primel
  • Sergiane S. Caldas
Full Short Communication


A rapid, simple and sensitive liquid chromatographic method has been developed to assay ciclopirox olamine in raw material and topical solution. The analysis used a reversed-phase C18 end-capped column, with UV detection at 305 nm and pre-column derivatization with dimethyl sulphate. A linear response (r 2 > 0.999) was observed in the range of 0.4–200 μg mL−1. The method showed good recoveries for the raw material and topical solution and the relative standard deviation intra and inter-day were ≤2.00%. Validation parameters such as specificity and robustness were also determined. The proposed method provided an accurate and precise analysis of ciclopirox olamine in raw material and topical solution.


Column liquid chromatography Derivatization and validation Ciclopirox olamine 



The authors would like to thank the Comissão Permanente de Revisão da Farmacopéia Brasileira for the financial support.


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Ana Laura V. Escarrone
    • 1
  • Celso F. Bittencourt
    • 1
  • Luciane V. Laporta
    • 2
  • Marcos R. dos Santos
    • 2
  • Ednei G. Primel
    • 3
  • Sergiane S. Caldas
    • 3
  1. 1.Programa de Pós-Graduação em Ciências FarmacêuticasUniversidade Federal de Santa Maria, UFSMSanta Maria-RSBrazil
  2. 2.Centro Universitário Franciscano, UNIFRASanta Maria-RSBrazil
  3. 3.Fundação Universidade de Rio Grande, FURGRio Grande-RSBrazil

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