LC Analysis of Lignans from Schisandra sphenanthera Rehd. et Wils.
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Schisandra sphenanthera Rehd. et Wils. is widely used in traditional Chinese medicine. A rapid and convenient method to separate and quantify four lignans (schisandrin, schisantherin A, deoxyschizandrin, and γ-schizandrin) was established by reversed-phase liquid chromatographic. On a Shimadzu C18 column (Phenomenex, 150 × 4.6 mm; 5 μm particle size), an isocratic flow elution program and a simplified sample pretreatment approach were used in the experiment. Samples from different parts of S. sphenanthera were extracted by chloroform and then separated with methanol and deionized water (70:30 v/v) at a flow rate of 0.8 mL min−1. The detection wavelength was set at 280 nm. The content of lignans in fruits is the highest, and the quantities of schisantherin A, deoxyschizandrin, and γ-schizandrin from fruits are 0.56, 0.54 and 0.30%, respectively. Schisandrin is not detected in all the plant extracts. This research forms a basic framework for the better use of S. sphenanthera in medicine.
KeywordsColumn liquid chromatography Lignans Schisandra sphenanthera Rehd. et Wils.
This work is supported by the National Eleventh-Five Year Science and Technology Support Program from Ministry of Science and Technology of the People’s Republic of China (No. 2006BAI06A13-06). Authors wish to acknowledge Dr. Xinbing Yang, Shuanghong Song, and Yaya Huang for technical help on LC. They would also like to thank colleagues in the Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry for their cooperation in carrying out this work.
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