, Volume 67, Issue 11–12, pp 979–983 | Cite as

LC Analysis of Lignans from Schisandra sphenanthera Rehd. et Wils.

  • Wei Gu
  • Nanyu Wei
  • Zhezhi Wang
Full Short Communication


Schisandra sphenanthera Rehd. et Wils. is widely used in traditional Chinese medicine. A rapid and convenient method to separate and quantify four lignans (schisandrin, schisantherin A, deoxyschizandrin, and γ-schizandrin) was established by reversed-phase liquid chromatographic. On a Shimadzu C18 column (Phenomenex, 150 × 4.6 mm; 5 μm particle size), an isocratic flow elution program and a simplified sample pretreatment approach were used in the experiment. Samples from different parts of S. sphenanthera were extracted by chloroform and then separated with methanol and deionized water (70:30 v/v) at a flow rate of 0.8 mL min−1. The detection wavelength was set at 280 nm. The content of lignans in fruits is the highest, and the quantities of schisantherin A, deoxyschizandrin, and γ-schizandrin from fruits are 0.56, 0.54 and 0.30%, respectively. Schisandrin is not detected in all the plant extracts. This research forms a basic framework for the better use of S. sphenanthera in medicine.


Column liquid chromatography Lignans Schisandra sphenanthera Rehd. et Wils. 



This work is supported by the National Eleventh-Five Year Science and Technology Support Program from Ministry of Science and Technology of the People’s Republic of China (No. 2006BAI06A13-06). Authors wish to acknowledge Dr. Xinbing Yang, Shuanghong Song, and Yaya Huang for technical help on LC. They would also like to thank colleagues in the Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry for their cooperation in carrying out this work.


  1. 1.
    Committee of National Pharmacopoeia (2005) Pharmacopoeia of the People’s Republic of China. Chemical Industry Press, Beijing, pp 48–49, 169–170Google Scholar
  2. 2.
    Committee of Flora of China (1996) Flora of China. Science Press, Beijing, 30(1), pp 252–260Google Scholar
  3. 3.
    Zhu M, Lin KF, Yeung RY, Li RC (1999) J Ethnopharmacol 67:61–68CrossRefGoogle Scholar
  4. 4.
    Kuo YH, Li SY, Wu MD, Huang RL, Kuo YLM, Chen CF (1999) Chem Pharm Bull 47:1047–1048Google Scholar
  5. 5.
    Kuo YH, Li SY, Huang RL, Wu MD, Huang HC, Lee KH (2001) J Nat Prod 64:487–490CrossRefGoogle Scholar
  6. 6.
    Liu JS, Huang MF, Yong T (1988) Can J Chem 66:414–415CrossRefGoogle Scholar
  7. 7.
    Chen DF, Zhang SX, Wang HK, Zhang SY, Sun QZ, Cosentino LM, Lee KH (1999) J Nat Prod 62:94–97CrossRefGoogle Scholar
  8. 8.
    Avula B, Choi YW, Srinivas PV, Khan IA (2005) J Chromatogr 61:515–518CrossRefGoogle Scholar
  9. 9.
    Tian K, Qi SD, Cheng YQ, Chen XG, Hu ZD (2005) J Chromatogr A 1078:181–187CrossRefGoogle Scholar
  10. 10.
    Deng CH, Song GX, Hu YM, Zhang XM (2003) J Chromatogr 58:289–294Google Scholar
  11. 11.
    Peng JY, Fan GR, Qu LP, Zhou X, Wu YT (2005) J Chromatogr A 1082:203–207CrossRefGoogle Scholar
  12. 12.
    Huang TH, Shen PN, Shen YJ (2005) J Chromatogr A 1066:239–242CrossRefGoogle Scholar
  13. 13.
    Wang YH, Gao JP, Chen DF (2003) China J Chin Mater Med 28:1155–1160Google Scholar
  14. 14.
    Zhu M, Chen XS, Wang KX (2007) J Chromatogr 66:125–128CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  1. 1.Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical ChemistryShaanxi Normal UniversityXi’anPeople’s Republic of China
  2. 2.College of Life SciencesShaanxi Normal UniversityXi’anPeople’s Republic of China

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