Chromatographia

, Volume 67, Issue 11–12, pp 941–945 | Cite as

Simultaneous Determination of Pyridoxine, Meclizine and Buclizine in Dosage Formulations and Human Serum by RP-LC

  • Muhammad S. Arayne
  • Najma Sultana
  • Farhan A. Siddiqui
Full Short Communication

Abstract

Rapid liquid chromatographic procedures for analytical quality control of pharmaceutical preparations and human serum containing antihistamine drugs, meclizine and buclizine alone or in combination with pyridoxine are proposed, using acetonitrile:water (80:20) as a mobile phase (pH adjusted to 2.6), methylparaben as internal standard and UV detection was made at 230 nm. The results obtained showed a good agreement with the declared content. The method shows good linearity in the range of 30–10,000 ng mL−1 for pyridoxine and 25–10,000 ng mL−1 for meclizine and buclizine serum concentrations with a correlation coefficient 0.9999 (inter- and intra-day CV < 3.91%). The recovery was >97.8%. The proposed method may be used for the quantitative analysis of meclizine and buclizine alone or in combination with pyridoxine from raw materials, in bulk drugs, dosage formulations and in serum.

Keywords

Column liquid chromatography UV detection Pyridoxine, meclizine and buclizine 

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Muhammad S. Arayne
    • 1
  • Najma Sultana
    • 2
  • Farhan A. Siddiqui
    • 1
  1. 1.Department of ChemistryUniversity of KarachiKarachiPakistan
  2. 2.Department of Pharmaceutical Chemistry, Faculty of PharmacyUniversity of KarachiKarachiPakistan

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